ABSTRACT
Background:
Differentiation of urothelial hyperplasia, dysplasia and carcinoma in situ (CIS) may pose diagnostic
difficulties. We aim to evaluate the role of CK20, p53 and p63 in
differentiation of such lesions. Methods: we evaluate these markers in 213
cases of bladder lesions (14 hyperplasia, 7 dysplasia, 5 CIS, 25 noninvasive
and 162 invasive urothelial carcinoma) in a retrospective study on sections
from formalin-fixed, paraffin-embedded blocks. Cytoplasmic staining considered
for CK20 and nuclear staining for p53 and p63. Results: CK20 was expressed in
14 out of 14 hyperplasia (4 strong, 7 moderate, 3 weak), in 7 out of 7
dysplasia (4 strong , 3 moderate), in 5 out of 5 CIS, in 19 out of 25
noninvasive carcinoma; strong in 11 (7 low grade, 4 high grade), moderate in 7
(5 low grade, 2 high grade), and weak in 1 case of low grade, in 136 out of 162
invasive carcinoma; strong in 46 (22 grade II, 24 grade III), moderate in 71 (2
grade I, 58 grade II, 11 grade III), and weak in 19 (2 grade I, 15 grade II, 2
grade III), no expression in 2 cases of grade IV. p53 was expressed in 7 out of
14 hyperplasia (weak in all cases), in 7 out of 7 dysplasia (5 strong, 2
moderate), in 5 out of 5 CIS (strong in all cases), in 20 out of 25 noninvasive
carcinoma (8 strong, 9 moderate, 3 weak), in 135 out of 162 invasive carcinoma
(34 strong, 73 moderate, 28 weak). p63 was expressed in 14 out of 14
hyperplasia, in 7 out of 7 dysplasia, in 4 out of 5 CIS, in 25 out of 25
noninvasive carcinoma, and in 134 out of 162 invasive carcinoma (28 strong, 63
moderate, 43 weak). CK20 was insignificant in noninvasive and significant in
invasive carcinoma, while p53 and p63 were significant in noninvasive and
invasive carcinoma. Conclusion: CK20, p53 and p63 are useful in differentiation
of different bladder lesions.
Share and Cite:
Raheem, S. , Saied, A. , Shaer, R. , Mustafa, O. and Ali, A. (2014) The Role of CK20, p53 and p63 in Differentiation of Some Urothelial Lesions of Urinary Bladder, Immunohistochemical Study.
Open Journal of Pathology,
4, 181-193. doi:
10.4236/ojpathology.2014.44024.