Author(s)

Te-Hua Hsu¹, Yue Ning², Jin-Chywan Gwo^1*

¹Department of Aquaculture, National Taiwan Ocean University, Keelung City.
²Fisheries Research Institute of Fujian, Xiamen, China.

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present a cheap and convenient AFLP-based (Amplified fragment length polymorphism) strategy that is highly efficient for developing informative SNP markers without any prior information. We developed SNP markers in a non-model and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential use of the combinations of AFLP and AFLP-SSCP.

AFLP, SNPs, Molecular Marker, SSCP

Hsu, T. , Ning, Y. and Gwo, J. (2014) AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms. Advances in Biological Chemistry, 4, 376-381. doi: 10.4236/abc.2014.46042.