American Journal of Analytical Chemistry

Volume 5, Issue 11 (August 2014)

ISSN Print: 2156-8251   ISSN Online: 2156-8278

Google-based Impact Factor: 1.71  Citations  h5-index & Ranking

Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

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DOI: 10.4236/ajac.2014.511081    16,755 Downloads   27,442 Views  Citations

ABSTRACT

Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H2O2 in different plant tissues and overcomes interference of soluble antioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.

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Junglee, S. , Urban, L. , Sallanon, H. and Lopez-Lauri, F. (2014) Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide. American Journal of Analytical Chemistry, 5, 730-736. doi: 10.4236/ajac.2014.511081.

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[195] Interactive effects of As, Cd and Zn on their uptake and oxidative stress in As-hyperaccumulator Pteris vittata
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[198] Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material
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[199] Sodium chloride primes JA-independent defense against Spodoptera litura (Fabricius) larvae in Arabidopsis thaliana
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[202] Fructan and antioxidant metabolisms in plants of Lolium perenne under drought are modulated by exogenous nitric oxide
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[203] Dynamic trajectories of volatile and non‐volatile specialised metabolites in 'overnight' fragrant flowers of Murraya paniculata
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[204] Roles of Hfq-Dependent sRNAs in E. amylovora Regulation of Virulence
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[205] Leaf age and light quality influence the basal resistance against Botrytis cinerea in strawberry leaves
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[209] Histochemical and Biochemical Alterations in the Stigma of Hibiscus syriacus (Malvaceae) During Flower Development
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[210] Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis
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[211] Impact of heat and drought stress on peroxisome proliferation in quinoa
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[212] Assessment of Oxidative Stress Responses in the Rare Species" Urginea Maritima in Bentael Natural Reserve Exposed to Anthropogenic Activities
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[213] Impact of exogenous spermine application on the vase life of cut rose flowers' Dolce Vita'.
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[214] Mycorrhiza Fungus Rhizophagus intraradices Mediates Drought Tolerance in Eleusine coracana Seedlings
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[215] Changes in sugar metabolism associated to stem bark thickening partially assist young tissues of Eriobotrya japonica seedlings under boron stress
Journal of Plant Physiology, 2018
[216] Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis
Biochemical and Biophysical Research Communications, 2018
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Environmental Science and Pollution Research, 2018
[218] Kaolin particle film modulates morphological, physiological and biochemical olive tree responses to drought and rewatering
Plant Physiology and Biochemistry, 2018
[219] A Growth-Promoting Bacteria, Paenibacillus yonginensis DCY84T Enhanced Salt Stress Tolerance by Activating Defense-Related Systems in Panax ginseng
Frontiers in plant science, 2018
[220] Systemic changes in photosynthesis and reactive oxygen species homeostasis in shoots of Arabidopsis thaliana infected with the beet cyst nematode Heterodera …
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[221] The synergistic effect of nickel-iron-foam and tripolyphosphate for enhancing the electro-Fenton process at circum-neutral pH
Chemosphere, 2018
[222] Differential Metal Tolerance and Accumulation Patterns of Cd, Cu, Pb and Zn in the Liverwort Marchantia polymorpha L.
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[223] Antioxidant capacity along the leaf blade of the C3-CAM facultative bromeliad Guzmania monostachia under water deficit conditions
2018
[224] Biochemical profile of non-enzymatic stress markers in the plant species “Urginea maritima” in a Mediterranean natural reserve exposed to oxidative stress
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[225] Determination of the expression level of stress-related genes in Cicer arietinum root cell under Cd stress and the relationship to H2O2 concentrations
Ecotoxicology, 2018
[226] ACCEPTED AUTHOR VERSION OF THE MANUSCRIPT
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Ecotoxicology and Environmental Safety, 2018
[228] Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate
Protoplasma, 2018
[229] Salicylic acid-induced germination, biochemical and developmental alterations in rye (Secale cereale L.)
Acta Botanica Croatica, 2018
[230] Mycorrhiza Fungus Rhizophagus intraradices Mediates Drought Tolerance in Eleusine coracana Seedlings 2
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[232] Iron-foam as a heterogeneous catalyst in the presence of tripolyphosphate electrolyte for improving electro-Fenton oxidation capability
Electrochimica Acta, 2018
[233] Oxidative stress response to aluminum oxide (Al2O3) nanoparticles in Triticum aestivum
Biologia, 2018
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Plant Growth Regulation, 2018
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[237] Effects of Increased Temperature and Copper Excess on the Physiology, Biochemistry and Gene Expression of Ectocarpus Siliculosus (Dillwyn) Lyngbye
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[238] Phytochemical, antioxidant and a study of bioactive compounds from Artemisia pallens
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[239] A growth promoting bacteria, Paenibacillus yonginensis DCY84T enhanced salt stress tolerance by activating defense-related systems in Panax ginseng
2018
[240] Impact of Exogenous Spermine Application on the Vase Life of Cut Rose Flowers 'Dolce Vita'
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[241] Oxidative stress response to aluminum oxide (Al2O3) nanoparticles in Triticum aestivum.
2018
[242] The effect of drought stress on some morphological and physiological characters in canola seedling (Brassica napus)
2018
[243] bstract
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2017
[246] Levelek alkalmazkodása a napfényhez–polifenolos vegyületek lehetséges szerepei
2017
[247] Induced Abiotic Stress: Prospects for Powdery Mildew Resistance in Tomato Plants
2017
[248] Exogenous aminolevulinic acid protects wheat seedlings against boron-induced oxidative stress
2017
[249] Adjustments in CAM and enzymatic scavenging of H 2 O 2 in juvenile plants of the epiphytic bromeliad Guzmania monostachia as affected by drought and rewatering
Plant Physiology and Biochemistry, 2017
[250] Endophytic Fungi Piriformospora indica Mediated Protection of Host from Arsenic Toxicity
Frontiers in microbiology, 2017
[251] A comparison of colorimetric assays detecting hydrogen peroxide in leaf extracts
Analytical Methods, 2017
[252] Prolonged culture of Boesenbergia rotunda cells reveals decreased growth and shoot regeneration capacity
Plant Cell, Tissue and Organ Culture (PCTOC), 2017
[253] Chrysanthemum morphology, photosynthetic efficiency and antioxidant capacity are differentially modified by light quality
Journal of Plant Physiology, 2017
[254] Adjustments in CAM and enzymatic scavenging of H2O2 in juvenile plants of the epiphytic bromeliad Guzmania monostachia as affected by drought and rewatering
Plant Physiology and Biochemistry, 2017
[255] Muscilage characterization, biochemical and enzymatic activities of laser irradiated Lagenaria siceraria seedlings
Journal of Photochemistry and Photobiology B: Biology, 2017
[256] 硫酸銅與低溫貯藏對台農 17 號鳳梨果實物化性狀與內部褐化之影響
臺灣中興大學學位論文, 2017
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Plant Cell Reports, 2017
[258] Mitochondrial uncoupling protein 1 overexpression increases yield in Nicotiana tabacum under drought stress by improving source and sink metabolism
Frontiers in Plant Science, 2017
[259] Abiotic Stress Response to As and As+ Si, Composite Reprogramming of Fruit Metabolites in Tomato Cultivars
Frontiers in Plant Science, 2017
[260] Polyethylene glycol (PEG) induced water stress alters the physiological and molecular responses of chia plants
2017
[261] Análisis del papel de la proteína EPL1 de Trichoderma atroviride en la resistencia a patógenos en Arabidopsis thaliana
2017
[262] Light quality effects on anatomy and physiology of ornamentals differing in their photosynthetic pathways
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[263] Can the damage caused by anthropogenic activities on Urginea maritima in Bentael natural reserve be a signal of health problems?
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[264] Assessment of Oxidative Stress Responses in The Rare Species” Urginea maritima” in Bentael Natural Reserve Exposed to Anthropogenic Activities
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[265] Ozonated water reduces susceptibility in tomato plants to Meloidogyne incognita by the modulation of the antioxidant system
Molecular Plant Pathology, 2016
[266] A novel procedure to assess the non-enzymatic hydrogen-peroxide antioxidant capacity of metabolites with high UV absorption
2016
[267] Ozonated water reduces susceptibility in tomato plants against Meloidogyne incognita by the modulation of the antioxidant system
Molecular plant pathology, 2016
[268] The physiological status and stress biomarker concentration of Coriandrum sativum L. plants subjected to chilling are modified by biostimulant application
Biological Agriculture & Horticulture, 2016
[269] Higher Activities of Defense-Associated Enzymes may Contribute to Greater Resistance of Manchurian Ash to Emerald Ash Borer Than A closely Related and Susceptible Congener
Journal of Chemical Ecology, 2016
[270] A kolinerg és glutamáterg neurotranszmisszió interakciójának vizsgálata kognitív zavarok állatkísérletes modelljeiben
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[271] Nostoc sp. extract induces oxidative stress-mediated root cell destruction in Mimosa pigra L.
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[272] Plant Functional Biology
[273] Aplikasi Pupuk Silika untuk Meningkatkan Ketahanan Tanaman Cabai Rawit (Capsicum annuum L.) terhadap Stres Genangan The Application of Silica …
[274] Environmental and Sustainability Indicators
[275] Towards characterization of Eurasian crop fruit resources: biochemical marker profiles in Azerbaijani and Iranian pomegranate cultivars under cold stress
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