Elevated Plasma Levels but Decreased Platelet-Associated Expression of LIGHT in Patients with Acute Atherothrombotic Stroke ()
Affiliation(s)
Department of Clinical Science, Intervention and Technology, Karolinska Institute, Stockholm, Sweden.
Department of Neurology, Peking University People’s Hospital, Beijing, China.
Key Laboratory Centre, Peking University People’s Hospital, Beijing, China.
ABSTRACT
Background: As a member of the tumor necrosis factor superfamily (TNFSF), LIGHT (TNFSF14) is expressed by a variety of immune cells
and exists in membrane-bound and soluble forms. Recently, LIGHT was found to be
associated with platelets and released upon activation. Activation of endothelia
cells by recombinant LIGHT protein results in pro-inflammatory and
pro-thrombotic changes. Several studies have reported increased plasma levels
of LIGHT in patients with stroke and cardiovascular diseases. However, the
form-associated roles of LIGHT in ischemic atherosclerotic stroke remain unclear. Mater?als and Methods: In this study, the
platelet LIGHT expression and soluble LIGHT protein were analyzed by flow
cytometry and enzyme-linked immunosorbent assay (ELISA) in peripheral blood of
patients with acute ischemic atherosclerotic stroke, asymptomatic carotid
stenosis (ACS) and normal controls. RESULTS: During the initial 24 h
after onset, the stroke patients had decreased LIGHT expression on their
platelets (5.9% ± 4.9%) and increased plasma LIGHT levels (36.1 ± 21.0 pg/ml) as compared with normal
controls (9.5% ± 3.0%, p < 0.05; 20.4 ± 13.4 pg/ml, p < 0.05).
Moreover, the platelet LIGHT expression correlated with total plaque area in
the stroke patients (r = 0.4572, p = 0.0247). Conclus?ons: The dysregulated LIGHT
expression reflects a persistent chronic inflammatory response that may have
been induced during early stages of ischemic atherosclerotic stroke. Our
results strongly suggest distinctive roles of form-associated LIGHT in the
disease pathogenesis: platelet-associated LIGHT may contribute to formation and
development of carotid atherosclerotic plaque, probably involving plaque
destabilization, while soluble LIGHT may predominantly functions as a
pro-inflammatory cytokine in the inflammatory process.
Share and Cite:
Liu, G. , He, Y. , Yang, T. , Jiang, H. , Xiang, Y. , Zhang, Q. , He, X. , Hjelmstrom, P. and Gao, X. (2014) Elevated Plasma Levels but Decreased Platelet-Associated Expression of LIGHT in Patients with Acute Atherothrombotic Stroke.
World Journal of Neuroscience,
4, 385-393. doi:
10.4236/wjns.2014.44042.
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