Author(s)

Nickolaj F. Starodub, Andrey M. Katsev, Elvira R. Abduramanova, Sergey L. Safronyuk, Marina V. Taran

Department of General Chemistry, Crimean State Medical University Named after S. I. Georgievsky, Simpheropol, Ukraine.
National University of Life and Environmental Sciences of Ukraine, Kiev, Ukraine.

Bioassay based on the luminescent bacteria was used as a new method for the determination of the kinetic constants of ligand binding to the complexion agent. Miramistin as the cationic surface-active antiseptic and serum blood albumin was taken as a model of interacting agent’s. It was concluded that this approach might have a wide range for applications, namely, in all cases where the ligand is an inhibitor of bacterial luminescence and its complex dose not effect the intensity of this process.

Bioluminescent Inhibition Test, Miramistin, Albumin, Association Constants, Determination

Starodub, N. , Katsev, A. , Abduramanova, E. , Safronyuk, S. and Taran, M. (2014) Determination of Binding Parameters of Miramistin to Serum Albumin by the Bioluminescent Method. Advances in Biological Chemistry, 4, 331-338. doi: 10.4236/abc.2014.45038.