Objective: To explore the effects of Anwei decoction (AD) on the protein expression of TFF in rats with chronic atrophic gastritis(CAG). Methods: Forty-eight healthy rats were randomly divided into 4 groups: normal control group, pathologic model group, Anwei Decoction group, and Weifuchun group. CAG was induced in rats with N-methy-N-nitro-N-nitrogua-nidine (MNNG). The protein expression of TFF in rats’ gastric mucosa was determined by immunohistochemistry. Results: Compared with that in the normal control group, the protein expression of TFF1 was significantly enhanced in the pathologic model, Anwei Decoction and Weifuchun groups (both P < 0.01). The protein expression level of TFF1 was significantly higher in the Anwei Decoction group than in the Weifuchun group (P < 0.01). Compared with the normal control group, the protein expression of TFF2 was significantly enhanced in the pathologic model, Anwei Decoction and Weifuchun groups (both P < 0.01). The protein expression level of TFF2 was significantly higher in the Anwei Decoction group than in the Weifuchun group (P < 0.01). In comparison with the pathologic model group, the protein expression of TFF3 was remarkably reduced in Anwei Decoction and Weifuchun groups (both P < 0.01). but there was no difference between the group of Anwei decoction and the group of Weifuchun (P > 0.05). Conclusion: Anwei decoction may be effective in the treatment of CAG by enhancing the protein expression of TFF1, TFF2 while reducing that of TFF3 in gastric mucosas.