Rapid Detection Method for Enteroaggregative Escherichia coli Using Simple Clump Formation and Aggregative Assay ()
ABSTRACT
Enteroaggregative Escherichia
coli (EAggEC) strains cause the persistent diarrhea in infants and
compromised hosts in developing countries. These strains are currently defined
as E. coli that adheres to HEp-2
cells in an aggregative adherence (AA) pattern. In this study, we compared 4
different rapid methods for the detection of EAggEC using a PCR assay, clump
formation test, glass slide adherence assay, and the HEp-2 cell adherence
assay. Out of 683 E. coli strains
isolated from diarrheal stool samples, we detected 17 aggR and/or clump-positive strains, and identified 2 aggR-positive, clump-negative strains
and 2 aggR-negative, clump-positive
strains. All the aggR positive and
clump positive strains also showed positive results in glass slide adherence
and HEp-2 cell adherence assays. From all these results, we suggest the
following procedure for the rapid identification of EAggEC strains: first,
screen E. coli strains with the clump
formation test and subsequently perform the glass slide adherence assay to
observe AA for confirmation.
Share and Cite:
M. Fujioka, C. Ahsan and Y. Otomo, "Rapid Detection Method for Enteroaggregative
Escherichia coli Using Simple Clump Formation and Aggregative Assay,"
Advances in Microbiology, Vol. 3 No. 8, 2013, pp. 552-556. doi:
10.4236/aim.2013.38074.