Author(s)

Mrinal Pal, Subinay Datta, Amit Kumar Pradhan, Lekha Biswas, Joydeep Ghosh, Partha Mondal, Rajarshi Rahut, Arghya Ray Chaudhuri, Sourav Sau, Soumi Das

Department of Biochemistry, Burdwan Medical College, Burdwan, India.
Department of Radiotherapy, Burdwan Medical College, Burdwan, India.

Background: Vitamin D is not only important for bone health but can also affect the development of several non-bone diseases. These findings have increased the need for determining vitamin D status in a convenient and cost-effective way. In order to determine the better method for detecting vitamin D status, we have estimated blood vitamin D in three methods such as high-pressure liquid chromatography, chemiluminescent immunoassays and enzyme immunoassays. Method: Two hundred and sixteen subjects irrespective of age and sex were studied for blood vitamin D in all the three methods over a period of 2 years. Result: The statistical analysis showed that values of vitamin D obtained by chemiluminescent immunoassays (r = 0.978, p < 0.001) were more significantly correlated with high-pressure liquid chromatography than enzyme immunoassays (r = 0.970, p < 0.001). Conclusion: We conclude that chemiluminescent immunoassays should be used instead of enzyme immunoassay and high-pressure liquid chromatography for estimation of vitamin D.

Vitamin D; High-Pressure Liquid Chromatography; Chemiluminescent Immunoassays; Enzyme Immunoassays

Pal, M. , Datta, S. , Pradhan, A. , Biswas, L. , Ghosh, J. , Mondal, P. , Rahut, R. , Chaudhuri, A. , Sau, S. and Das, S. (2013) Comparison between different methods of estimation of vitamin D. Advances in Biological Chemistry, 3, 501-504. doi: 10.4236/abc.2013.35054.