Genetic Variation among various populations of spadefoot toads (Pelobates syriacus, Boettger, 1869) at breeding sites in northern Israel - Advances in Biological Chemistry

ABC > Vol.3 No.5, October 2013

Volume 3, Issue 5 (October 2013)

ISSN Print: 2162-2183 ISSN Online: 2162-2191

Google-based Impact Factor: 0.5 Citations

Genetic Variation among various populations of spadefoot toads (Pelobates syriacus, Boettger, 1869) at breeding sites in northern Israel ()

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DOI: 10.4236/abc.2013.35047 3,680 Downloads 6,479 Views Citations

Author(s)

Gad Degani

Affiliation(s)

School of Science and Technology, Tel Hai Academic College, Upper Galilee, Israel.

ABSTRACT

A genetic study was carried out on spadefoot toads (Pelobates syriacus) from habitats of various locations and altitudes in northern Israel. Cytochrome b and 12S were amplified by PCR for the analysis of genetic variation based on five DNA polymorphisms and for RAPD PCR. The nucleotide sequences of the mitochondrial DNA fragments were determined from a 460 bp clone of cytochrome b and a 380 bp clone of 12S (GenBank accession numbers, FJ595199-FJ59-5203). No genetic variation was found among the populations with regard to 12S. According to the analysis of five sequences using Arlequin software, there was a high gene identity among the populations (98.7%-99.6%). Both populations, Elrom Pond breeding site, at the highest altitude and Fara Pond, at the lowest, had the lowest identities as compared to other populations. The DNA variation among P. syri-acus populations from various breeding sites, according to band sharing (BS), when using the OP-4 primer, was 0.92-1.00. Similarity was low between the population of Elrom Pond and the populations of Kash Pond, Raihania Pond and Sasa Pond (0.92 BS), as well as between the population of Fara Pond at the lowest altitude, and the populations of Kash Pond, Raihania Pond and Sasa Pond (0.92 BS). Similar results were obtained when comparing the results obtained using primer OPA-3. The lowest similarity was found between populations of the highest altitude (Elrom Pond) and lowest altitude (Fara Pond), relative to the other ponds (Kash Pond, Raihania Pond and Sasa Pond) with a BS of 0.93.

KEYWORDS

Spadefoot Toads; Pelobates syriacus; Cytochrome b; 12S; DNA Fragments

Share and Cite:

Degani, G. (2013) Genetic Variation among various populations of spadefoot toads (Pelobates syriacus, Boettger, 1869) at breeding sites in northern Israel. Advances in Biological Chemistry, 3, 440-447. doi: 10.4236/abc.2013.35047.

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