Usefulness of Immuno-Magnetic Beads Conjugated with Anti-EpCAM Antibody for Detecting Endometrial Cancer Cells ()
Author(s)
Yoshikatsu Koga,
Satoshi Katayose,
Nobuko Onoda,
Takahiro Kasamatsu,
Tomoyasu Kato,
Shunichi Ikeda,
Mitsuya Ishikawa,
Ken Ishitani,
Yasuo Hirai,
Hideo Matsui,
Yasuhiro Matsumura
Affiliation(s)
Department of Gynecology, National Cancer Center Hospital, Tokyo, Japan.
Department of Obstetrics and Gynecology, Tokyo Women’s Medical University, Tokyo, Japan.
Department of Obstetrics and Gynecology, Tokyo Women’s Medical University, Tokyo, Japan..
Division of Developmental Therapeutics, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa, Japan.
R & D Department Unit 1, JSR Life Sciences Corporation, Tsukuba, Japan.
ABSTRACT
A simple and non-invasive
method for detecting endometrial cancer in women with abnormal uterine bleeding
is required. For this
purpose, we prepared immuno-magnetic beads conjugated with anti-human EpCAM rat
monoclonal antibody (mAb) for isolating exfoliated endometrial cells including
endometrial cancer cells in vaginal discharge. The affinities of the anti-human
EpCAM rat mAbs were analyzed by flow cytometry and immunocytochemistry and then
magnetic beads were conjugated with the mAbs. The rate of retrieval of
endometrial cells using the immuno-magnetic beads was calculated. Endometrial
cells were isolated using the immuno-magnetic beads from the vaginal discharges
of 22 patients with endometrial cancer and 16 non-malignant controls. The
isolated cells were stained using endometrial cancer specific-mAbs and analyzed
by flow cytometry and imaging cytometry. The immuno-magnetic beads conjugated
with high-affinity mAb (clone 1456) appeared to have very low auto-fluorescence. Sufficient enrichment
of Ep-CAMpositive cells
using immuno-magnetic beads was observed in both simulation and clinical
samples. The overall sensitivities of flow cytometry and imaging cytometry to
detect endometrial cancer cells were 72.7% and 45.5%, respectively. Meanwhile,
the overall specificities of flow cytometry and imaging cytometry for healthy
controls were 75.0% and 81.3%, respectively. Our immuno-magnetic beads have
very low auto-fluorescence, so they could be useful for fluorescent analysis,
such as fluorescent immunochemical staining. In the future, these novel
immuno-magnetic beads could be used for cytological study.
Share and Cite:
Y. Koga, S. Katayose, N. Onoda, T. Kasamatsu, T. Kato, S. Ikeda, M. Ishikawa, K. Ishitani, Y. Hirai, H. Matsui and Y. Matsumura, "Usefulness of Immuno-Magnetic Beads Conjugated with Anti-EpCAM Antibody for Detecting Endometrial Cancer Cells,"
Journal of Cancer Therapy, Vol. 4 No. 8, 2013, pp. 1273-1282. doi:
10.4236/jct.2013.48150.