In order to meet the requirements of Decree of the State Council of the People's Republic of China (No.599), this paper presents a three-defense line coordination strategy based on the Decree of the State Council (No.599). Taking the Guizhou power grid of 220 kV&500 kV as an example, the security and stability problem of the Guizhou power grid is analyzed. The strategy using three-defense line coordination for lowering the level of electricity safety accidents is researched. The analysis results show that the proposed coordination strategy is feasible, which can lower the level of electricity safety accidents under the same fault in same operation mode. Through studying the three-defense line of the Guizhou power grid, this paper presents a basic idea of the three-defense line coordination strategy based on the Decree of the State Council (No.599). The three-defense line coordination strategy provides reference for other provinces power grid.