Author(s)

Zair S. Shakirov, Sardor A. Khakimov, Khabibullo F. Shomurodov, Bakhtiyar R. Umarov

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A total of 110 strains of nodule bacteria was isolated from plants Onobrychis transcaucasica and Onobrychis chorassanica. Nodulation study of bacteria in both Onobrychis plant species in microvegetation experiment gave a very low nodulation on plant roots. The intensive nodulation of Onobrychis plants was recorded in vegetation experiment and for Onobrychis transcaucasica the efficiently-nodulating strains were found OT102, OT103, OT117, OT121, OT130, OT136, ОT139, ОT140, while for Onobrychis chorassanica plants—ОC106, ОC107, ОC109, ОC112, ОT103, ОT117 and ОT123 strains. Nucleotide sequencing of the 16S rRNA gene and BLAST analysis showed that nodule bacteria of Onobrychis plants were related to Rhizobium, Burkholderia, Enterobacter and Pantoea genera. It has been shown a possibility of growing up of Onobrychis plants at minimal additional moisture of sabulous soils in the Kyzyl Kum Desert, creating artificial pastures and thereby immobilizing the desert blown sands.

Onobrychis Transcaucasica, Onobrychis Chorassanica, Nodulation, Nitrogen Fixation, 16S rRNA, Rhizobium, Burkholderia, Enterobacter, Pantoea

Z. Shakirov, S. Khakimov, K. Shomurodov and B. Umarov, "Nodulation in Onobrychis Perennial Legume Plants," American Journal of Plant Sciences, Vol. 1 No. 2, 2010, pp. 119-130. doi: 10.4236/ajps.2010.12016.