Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol - Journal of Biomaterials and Nanobiotechnology

JBNB > Vol.4 No.3, July 2013

Journal of Biomaterials and Nanobiotechnology

Volume 4, Issue 3 (July 2013)

ISSN Print: 2158-7027 ISSN Online: 2158-7043

Google-based Impact Factor: 1.69 Citations

Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol ()

HTML XML

Download as PDF (Size: 688KB) PP. 242-246

DOI: 10.4236/jbnb.2013.43030 3,092 Downloads 4,599 Views Citations

Author(s)

Nikita O. Ronzhin, Alexey V. Baron, Ekaterina S. Mamaeva, Alexey P. Puzyr, Vladimir S. Bondar

Affiliation(s)

Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia..

ABSTRACT

Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of enzymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes exhibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling (Н₂О₂—4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and various buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional activity after long storage at + 4°C.

KEYWORDS

Nanodiamond; Test-System; Glucose; Cholesterol

Share and Cite:

N. Ronzhin, A. Baron, E. Mamaeva, A. Puzyr and V. Bondar, "Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 3, 2013, pp. 242-246. doi: 10.4236/jbnb.2013.43030.

Cited by

[1]	EPR Spectrometric Estimation of the Distribution of Intravenously Injected Nanodiamonds in Mice
	2019

[2]	Оценка методом ЭПР-спектрометрии распределения в организме мышей внутривенно введенных наноалмазов
	2019

[3]	Production of a Composite Based on Alumina Nanofibers and Detonation Nanodiamonds for Creating Phenol Indication Systems
	2019

[4]	Fabrication of a composite based on aluminum oxide nanofibers and nanodiamonds to construct phenol detection systems
	2019

[5]	Получение композита на основе нановолокон оксида алюминия и наноалмазов для создания систем индикации фенола
	2019

[6]	A Supramolecular Indicator System for Detecting Urea Based on the Cellulose-Nanodiamond Composite Material and Urease
	2019

[7]	БИОМЕДИЦИНСКИЕ ПРИЛОЖЕНИЯ НАНОАЛМАЗОВ ВЗРЫВНОГО СИНТЕЗА
	2019

[8]	Конструирование надмолекулярной индикаторной системы" целлюлоза-наноалмаз-уреаза"
	2018

[9]	Nanodiamonds as an effective adsorbent for immobilization of extracellular peroxidases from luminous fungus Neonothopanus nambi to construct a phenol detection …
	Biocatalysis and Biotransformation, 2018

[10]	БИФУНКЦИОНАЛЬНАЯ ИНДИКАТОРНАЯ СИСТЕМА ДЛЯ ОПРЕДЕЛЕНИЯ ФЕНОЛА И ГЛЮКОЗЫ НА ОСНОВЕ НАНОАЛМАЗОВ И ЭКСТРАКЛЕТОЧНЫХ …
	2018

[11]	КОНСТРУИРОВАНИЕ НАДМОЛЕКУЛЯРНЫХ СИСТЕМ ИНДИКАЦИИ ПОЛИМЕРНЫЙ НОСИТЕЛЬ–НАНОАЛМАЗ–БИОМАРКЕР
	2018

[12]	ОЦЕНКА МЕТОДОМ ЭПР-СПЕКТРОСКОПИИ РАСПРЕДЕЛЕНИЯ НАНОАЛМАЗОВ В ОРГАНИЗМЕ МЫШЕЙ ПРИ ВНУТРИВЕННОМ ВВЕДЕНИИ
	2017

[13]	The Effects of Nanodiamonds at the Action of Colored Metal Ions on the Skin of Guinea Pigs
	2016

[14]	Designing a reusable system based on nanodiamonds for biochemical determination of urea
	Doklady Biochemistry and Biophysics, 2015

[15]	КОНСТРУИРОВАНИЕ МНОГОРАЗОВОЙ СИСТЕМЫ БИОХИМИЧЕСКОГО ОПРЕДЕЛЕНИЯ МОЧЕВИНЫ НА ОСНОВЕ НАНОАЛМАЗОВ
	2015

[16]	NANOTECHNOLOGIA W BIOMEDYCYNIE.
	2015

[17]	Связывание иммуноглобулинов сыворотки крови человека наноалмазами
	2014

[18]	Сравнительное исследование протекторного эффекта модифицированных наноалмазов взрывного синтеза при систематическом воздействии …
	2014

[19]	Binding the immunoglobulins of human serum by nanodiamonds
	Doklady Biochemistry …, 2014

[20]	SVYaZYVANIE IMMUNOGLOBULINOV SYVOROTKI KROVI ChELOVEKA NANOALMAZAMI
	DA Nauk - Doklady Akademii Nauk, 2014, 2014

[21]	Сравнительное исследование протекторного эффекта модифицированных наноалмазов взрывного синтеза при систематическом воздействии …
	2014

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies