Passion fruit has great prospective to fascinate fruit consumer for its taste and delicious fruit juice and improvement of the economic condition of the fruit grower in the developing countries. The self-incompatibility in the passion fruits is an imperative reason to be considered regarding fruit production. Pollination is an essential for self-sterile crops as passion fruit (Passiflora edulis Sims.). The experiment was conducted in the field and laboratories of the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh to investigate the fruit setting behavior of passion fruits at during five flashes. We estimated to study fruit setting behavior of passion fruit at different flashes and determine effective pollination method and suitable flashes among five flashes. Result revealed that percent of fruit set of passion fruit was recorded highest; Seeds per plant were recorded highest in third flash when flowers were pollinated by hand compared with self-, and natural pollinations. Length-breadth ratios of fruits in third flashes were recorded higher when pollinates by hand. Individual fruit weight was also recorded higher at third flash. Plants required minimum days from flower anthesis to full maturity during third flash. On the other hand, fruit growth behavior of hand pollinated flowers was recorded higher during third flash of passion fruit. Results indicated that all studied characteristics of fruit and seed of passion fruit of third flash performed best.