Occurrence of tetracycline residues and antimicrobial resistance in gram negative bacteria isolates from cattle farms in Spain - Advances in Bioscience and Biotechnology

ABB > Vol.4 No.2, February 2013

Advances in Bioscience and Biotechnology

Volume 4, Issue 2 (February 2013)

ISSN Print: 2156-8456 ISSN Online: 2156-8502

Google-based Impact Factor: 1.18 Citations h5-index & Ranking

Occurrence of tetracycline residues and antimicrobial resistance in gram negative bacteria isolates from cattle farms in Spain ()

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Download as PDF (Size: 458KB) PP. 295-303

DOI: 10.4236/abb.2013.42A040 5,705 Downloads 10,502 Views Citations

Author(s)

Matilde Carballo, Fernando Esperón, Carlos Sacristán, Miguel González, Belén Vázquez, Sonia Aguayo, Ana de la Torre

Affiliation(s)

National Centre for Environmental Health, Group of Water Pollution, Instituto de Salud Carlos III, Madrid, Spain.
Research Centre in Animal Health, CISA-INIA, Madrid, Spain.

ABSTRACT

The usage of antibiotics in animal husbandry has dramatically increased the concentration of antibiotic residues and has promoted the development and abundance of antibiotic resistance in manure. When it is spread onto agricultural land, both residues of antibiotics and bacteria carrying antibiotic resistance genes may be introduced into the environment. In this research, we isolated resistant gram negative bacteria from manure produced in two dairy and two beef cattle farms, located in Madrid (Spain), to determine their resistance to seventeen representative antibiotics commonly used in veterinary therapy. A total of 63 isolates were used to assess the overall bacterial antimicrobial resistance on cattle manure samples. Predominant species were Escherichia coli and Comamonas testosteroni accounting for 25% and 19.6% of the total, respectively. The most found antimicrobial resistances in gram-negative bacteria were to tetracycline (66.7%), sulphamethoxazole (55.6%), ampicillin (52.4%), cephalothin (46.0%), chloramphenicol (44.4%), nalidixic acid (39.7%) and trimethroprim- sulphamethoxazole (33.3%). The mean of resistance and the percentage of multi-resistant bacteria in beef farms were higher and statistically significant when compared to dairy farms which is opposite from the findings of the previous studies. The presence of three tetracyclines in all manure samples was also examined with stable recoveries (76% - 82%) and high sensitivity (limit of quantification 0.015 - 0.03 μg/kg). The concentrations of tetracyclines detected (<0.015 - 10 mg/kg) were consistent to the theoretical tetracycline levels in manure in Spain according to the ex- cretion rate of these antibiotics and the values re-ported in scientific literature in other European coun- tries.

KEYWORDS

Antimicrobial Resistance; Gram Negative Bacteria; Cattle Manure; Tetracycline

Share and Cite:

Carballo, M. , Esperón, F. , Sacristán, C. , González, M. , Vázquez, B. , Aguayo, S. and Torre, A. (2013) Occurrence of tetracycline residues and antimicrobial resistance in gram negative bacteria isolates from cattle farms in Spain. Advances in Bioscience and Biotechnology, 4, 295-303. doi: 10.4236/abb.2013.42A040.

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