Author(s)

Hoa Minh Luong, Sheng-Fan Wang, Vu Tan Trao, Marcelo Chen, Jason C. Huang, Phung Dac Cam, Yu-Ting Lin^*, Yi-Ming Arthur Chen^*

Division of Enteric Infections, Department of Bacteriology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
Immunology and Molecular Biology Department, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
National Supervising Council of Professor in Medical bBranch, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
AIDS Prevention and Research Center, National Yang-Ming University, Taipei City.
Department of Microbiology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung.
Institute of Biomedical Sciences, Academia Sinica, Taipei City.
Mackay Medicine, Nursing and Management College, Taipei City.

Outbreaks of highly pathogenic avian influenza H5N1 virus have occurred in Vietnam since 2003. However, how people got avian H5N1 infection in Northern Vietnam is still unclear. We therefore performed a combination of the serologic assays H5N1 ELISA and H5 western blot to detect anti-H5N1 specific antibodies. Sera samples of 149 subjects with suspected H5N1 infection from three provinces of Northern Vietnam were collected from September 2006 to March 2007. Our results indicated that this combinational assay showed high sensitivity (100%) and specificity (95%) when compared with hemagglutinin inhibition (HI) assay. Fifty-one sera samples (34.2%) contained specific antibodies against H5N1 viruses. Poultry raisers (32/77; 41.6%) showed higher H5N1 infection rates than slaughterers (12/41; 29.3%) and health care workers (7/31; 22.6%). Contact history with sick or dead poultry in household or slaughter-house (p < 0.05) and lack of protective equipment use when in contact with dead poultry (p < 0.05) were risk factors found to be associated with H5N1 infection. In this study, we established an alternative serologic assay for H5N1 diagnosis, and we hereby present seroepidemiologic data of H5N1 infection in Northern Vietnam.

H5N1; ELISA; Western Blot; Hemagglutinin Inhibition; Vietnam

Luong, H. , Wang, S. , Trao, V. , Chen, M. , Huang, J. , Cam, P. , Lin, Y. and Chen, Y. (2012) Retrospective survey of avian influenza H5N1 infection in Northern Vietnam by using a combinational serologic assay. Health, 4, 974-982. doi: 10.4236/health.2012.430149.