Author(s)

Dmitry E. Pugachev^1*, Georgy V. Zatonsky¹, Tatyana S. Kostryukova¹, Anna G. Shubina², Nikolay V. Vasiliev^1,2

¹Department of Biological Microanalysis, FGUP State Research Institute of Biological Engineering, Moscow, Russia.
²Department of Theoretical and Applied Chemistry, State University of Education, Mytishchi, Russia.

New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu³⁺ ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.

Fluorescence Immunoassay, Fluorinated β-Diketones, Carbazole, Europium Complexes, Streptavidin, Nanodispersions

Pugachev, D. , Zatonsky, G. , Kostryukova, T. , Shubina, A. and Vasiliev, N. (2024) 3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents. International Journal of Organic Chemistry, 14, 20-31. doi: 10.4236/ijoc.2024.141002.