Author(s)

Tangtang Song¹, Yinhui Yao¹, Yazhen Shang^1,2*

¹Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde, China.
²Hebei Province Key Research Office of Traditional Chinese Medicine against Dementia, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development, Hebei Key Laboratory of Nerve Injury and Repair, Chengde, China.

Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ_1-42-induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by Aβ_1-42, an injury model was established by subjecting OLN-93 cells to Aβ_1-42 exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ_1-42. The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate Aβ_1-42-induced damage of OL.

Flavonoids form the Stem and Leaves of Scutellaria baicalensis Georgi, Aβ_1-42, Oligodendrocytes, Damage

Song, T. , Yao, Y. and Shang, Y. (2024) The Protective Effects of Flavonoids from Scutellaria Baicalensis Georgi Stems and Leaves on Oligodendrocyte Damage Induced by Aβ_1-42. Pharmacology & Pharmacy, 15, 1-13. doi: 10.4236/pp.2024.151001.