Author(s)

Juan Fabian¹, Taylor Pierce², Shenell Brown², Jazmyne Smith¹, Dolapo Adedeji^2*, Gloria Payne¹

¹Department of Natural Sciences, Elizabeth City State University, Elizabeth City, NC, USA.
²Department of Health & Human Studies, Elizabeth City State University, Elizabeth City, NC, USA.

The combination of Artesunate (ART) and Paclitaxel (PTX) in two human prostate cancer (PCa) cell lines (PC-3 and LNCaP) was evaluated to investigate the effects on proliferation, apoptosis and morphological changes. The half maximal inhibitory concentration (IC₅₀) values that were observed by ART and PTX on both LNCaP and PC-3 cell lines at 72-and 120-hour exposure were used to assess these effects. Early and late apoptosis was detected in Annexin V-FITC/PI assay revealed a shift in population of cells towards early and mid-apoptosis with ART + PTX than with ART and PTX individually. More effects were observed on LNCaP cell lines at both 72-hour and 120-hour exposure. The results for the Caspase 3/7 activity assay showed shift of viable population in all induced samples compared to control. Morphological changes occurred in both cell lines; this was validated in qualitative assessment when examined under the inverted microscope. These findings indicated that ART + PTX suppressed PCa cell proliferation in a dose- and time-dependent manner.

Combination, Prostate Cancer, Artesunate, Paclitaxel, Anticancer Activities

Fabian, J. , Pierce, T. , Brown, S. , Smith, J. , Adedeji, D. and Payne, G. (2023) The Combination of Artesunate and Paclitaxel in 1:1 Ratio Induces Apoptosis and Morphology Change on Human Prostate Cancer Cell Lines. Pharmacology & Pharmacy, 14, 482-492. doi: 10.4236/pp.2023.1411032.