This study aimed to investigate the effect of fatigue characteristics on the static and dynamic performance of Eucommia ulmoides gum isolators, and to explore the performance changes of Eucommia ulmoides gum isolators with different formulations. For this purpose, we used five formulations of Eucommia ulmoides gum isolators and set different fatigue test methods to study the static and dynamic performance changes of Eucommia ulmoides gum isolators with different formulations by changing the amplitude. The experimental results showed that the addition of Eucommia ulmoides gum had an impact on the performance of the isolator, and the number of fatigue cycles would lead to the hardening of the Eucommia ulmoides gum isolator and changes in its static and dynamic performance. In the range of two million vibrations, the performance change of the isolator was significant in the early stage and then tended to be flat, indicating that the impact of fatigue on the performance of the isolator would not continue to persist. It is worth noting that the study found that the addition of 30% Eucommia ulmoides gum had the least impact on the performance of the isolator under fatigue. Therefore, for long-term use of Eucommia ulmoides gum isolators, attention should be paid to their fatigue characteristics to ensure their stability and reliability. Additionally, this study provides a reference for the design and application of Eucommia ulmoides gum isolators. In summary, this study provides important reference value for a deeper understanding of the fatigue characteristics of Eucommia ulmoides gum isolators and for ensuring their stable and reliable performance.