Author(s)

Sahar Abd Al-Daim

Water Pollution Research Department, Environment and Climate Change Institute, National Research Centre, Cairo, Egypt.

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.

DNA Agarose Electrophoresis Buffer, Tang Buffer, Bicarbonate Buffer, PCR, TAE Buffer, Supper Buffer

Al-Daim, S. (2023) Comparison between Four Types of Buffers for DNA Agarose Gel Electrophoresis. Journal of Biomaterials and Nanobiotechnology, 14, 47-52. doi: 10.4236/jbnb.2023.143004.