Author(s)

Sylvain Pechangou Nsangou¹, Charifa Ngbetkom Mandou¹, Carine Matsigang Fondjou¹, Vigny Sayal Ngohoba¹, Edie Brice Enang II¹, Ibrahim Njingou¹, Emmanuel Mfotie Njoya^1,2, Frederic Nico Njayou^1*, Paul Moundipa Fewou¹

¹Laboratory of Pharmacology and Toxicology Department of Biochemistry, Faculty of Sciences, University of Yaounde1, Yaounde Cameroon.
²Centre for Quality of Health and Living, Faculty of Health and Environmental Sciences, Central University of Technology, Bloemfontein, South Africa.

Codiaeum variegatum has been widely investigated for its biological proprieties ranging from the antiamoebic potential to the phytochemical analysis. The aim of the present study was to evaluate the anti-inflammatory potential of C. variegatum leaf extracts and fractions. A primary macrophage culture activated by Saccharomyces cereviseae (SC) was used to evaluate cell cytotoxicity and anti-inflammatory potential of the plant extracts and fractions. Macrophages were treated with different concentrations (0.1; 1; 10 and 100 μg/mL) of the extracts/fractions for the inhibition of 5-lipoxygenase activity, nitric oxide (NO) and Tumor Necrosis Factor Alpha (TNF-α) production. No significant difference was observed on cell viability in the presence of extracts and fractions at tested concentration during the incubation period. Extracts and fractions of C. variegatum inhibited the 5-lipoxygenase activity, NO and TNF-α production by viable primary mouse macrophages in a concentration-dependent manner. The fractionation process increased anti-inflammatory activity. Among fractions, HEF2, HEF3, HEF5, EEF1, EEF3 and EEF5 exhibited the best anti-inflammatory potential. C. variegatum extracts and fractions exhibited a greater anti-inflammatory potential throughout the inhibition of pro-inflammatory mediators such as NO, 5-Lox and TNF-α.

C. variegatum, Anti-Inflammatory, 5-Lipoxygenase, NO, TNF-α

Nsangou, S. , Mandou, C. , Fondjou, C. , Ngohoba, V. , Enang II, E. , Njingou, I. , Njoya, E. , Njayou, F. and Fewou, P. (2023) Codiaeum Variegatum Hydro Alcoholic Leaf Extracts and Their Fractions Inhibit Pro-Inflammatory Mediators in Vitro. Journal of Biosciences and Medicines, 11, 40-54. doi: 10.4236/jbm.2023.115004.