The paper puts forward a new educational model, namely computer assisted entrepreneurship education which is based on various cases of entrepreneurship talents cultivation in Chinese Universities. First it highlights computer database to serve cultural and economic development. Then it introduces entrepreneurship education to meet challenges through regression analysis method to predict the demand of Chinese entrepreneurs after university students’ graduation; next it analyzes practice for entrepreneurship education model and cites e-commerce major as an example; thereby it specifies reform in talents cultivation as well as entrepreneurship education in practice. Finally some evaluations and problems are given based on entrepreneurship education practices of Chinese Universities. Namely Chinese Universities will lay emphasis on entrepreneurial ability, combine talents cultivation with regional economy development, expand and promote cross-culture so as to achieve the fostering goal for interdisciplinary talents who will not only obtain employment and entrepreneurship but also serve regional economy.