Author(s)

Abdou Khadre Sané^1,2, Aboubacry Kane^2,3, Bassirou Diallo^2,4, Mariama Ngom^1,2,3, Djibril Sané¹, Mame Ourèye Sy^1,2*

¹Laboratoire Campus de Biotechnologies végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
²Laboratoire Mixte International-Adaptation des Plantes et Microorganismes associés aux Stress Environnementaux (LMI-LAPSE), IRD, ISRA, UCAD, Dakar, Sénégal.
³Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel Air, Dakar, Senegal.
⁴Laboratoire National de Recherches sur les Productions Végétales (LNRPV), Unité de recherche en culture in vitro (URCI), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, Sénégal.

Salinity is a major problem that seriously impacts agricultural production, particularly that of tomato (Solanum lycopersicum L.). However, the plant has the ability to associate with Arbuscular Mycorrhizal Fungi to better tolerate salt stress. Thus, thanks to the extension of the AMF hyphae, the hydromineral nutrition and the tolerance to excess toxic ions (Na⁺ and Cl^-) of the plant are optimized. In this context, the contribution of AMF to the salt stress tolerance of two tomato varieties under semi-controlled conditions was studied. To do this, the frequency and intensity of mycorrhization, the relative mycorrhizal dependency, the survival rates, the aerial and root dry weights, the mineral (P, K⁺, Na⁺) and proline contents of the plants subjected to four levels of salinity [0, 70, 140 and 210 mM of NaCl] were evaluated. All the parameters assessed appeared to be dependent on the variety, the fungal strain and the NaCl concentration. With the Lady Nema variety, inoculation with the Claroideoglomus etunicatum strain at [NaCl 140 mM] resulted in the highest frequencies (54%), intensities (40.47%), and relative mycorrhizal dependencies (19.65%). This same symbiotic couple recorded high survival rates (55%) and aerial (2.03 g) and root (0.50 g) dry weights. Significant contents of K⁺ (Leaves: 7.5 mg⋅g^-1; Roots: 4.4 mg⋅g^-1 of dry matter), P (Leaves: 15.15 mg⋅g^-1 of dry matter) and proline (975 nmoles⋅g^-1 of fresh matter) were also recorded by this pair, with the lowest Na⁺ contents (Leaves: 1.93 mg⋅g^-1; Roots: 0.96 mg⋅g^-1 of dry matter). For the Mongal variety, at [NaCl 140 mM], the highest frequencies (50.36%), intensities (35.14%) and relative mycorrhizal dependencies (43.95%) were obtained thanks to inoculation with Rhizophagus fasciculatus. The highest survival rates (59%) and aerial (2.58 g) and root (0.79 g) dry weights were also obtained with this symbiotic couple. The contents of K⁺ (Leaves: 6.1 mg⋅g^-1; Roots: 3.09 mg⋅g^-1 of dry matter), P (Leaves: 12.49 mg⋅g^-1 of dry matter) and proline (942 nmoles⋅g^-1 of fresh matter) the most important and those in Na⁺ the lowest (Leaves: 2.03 mg⋅g^-1; Roots: 1.53 mg⋅g^-1 of dry matter) were also recorded for this same pair. Thus, the best fungal partner for the Lady Nema variety is C. etunicatum, followed by F. mosseae and R. fasciculatus, while for the Mongal variety it is R. fasciculatus, followed by C. etunicatum and F. mosseae.

Solanum lycopersicum, Salt Stress, Arbuscular Mycorrhizal Fungi, Growth, Phosphorus, Potassium, Sodium, Proline, Tolerance

Sané, A. , Kane, A. , Diallo, B. , Ngom, M. , Sané, D. and Sy, M. (2022) Response to Inoculation with Arbuscular Mycorrhizal Fungi of Two Tomato (Solanum lycopersicum L.) Varieties Subjected to Salt Stress under Semi-Controlled Conditions. Agricultural Sciences, 13, 1334-1362. doi: 10.4236/as.2022.1312082.