Author(s)

Xin Huang¹, Xiaowei He^2*, Yirong Gan¹, Bing Wang¹, Man Zhang¹, Jianye Dai², Yuan Yu¹

¹The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, China.
²Guangxi University of Chinese Medicine, Nanning, China.

Objective: To investigate the mechanism of inhibiting the secretion of “lithogenic bile” and treating gallbladder distention with the TCM prescription Dahuang Lingxian. Methods: Forty SPF-grade SD male mice were randomly divided into blank control group, model group, ursodeoxycholic acid group and TCM group, 10 mice in each group. The blank control group was kept normally, and the model group, ursodeoxycholic acid group and TCM group were established as gallbladder distention models. The blank control group and the model group were given 1.5 ml of saline by gavage daily; the ursodeoxycholic acid group was given 100 mg/kg/d of a mixture of saline and ursodeoxycholic acid capsules by gavage daily; the TCM group was given 39 g/kg/d of aqueous decoction of Dahuang Lingxian Prescription by gavage daily. After 6 weeks of continuous intervention, all mice were executed and the lithogenesis rate, ultrastructure and mRNA expression were observed. Results: The lithogenesis rate of mice in the TCM group was significantly reduced (P < 0.0083). The cell ultrastructure showed that the nuclear membrane was intact, the endoplasmic reticulum and mitochondrial content were significantly increased, and the expression of key mRNAs in the TGF-β1/Smads pathway was significantly improved (P < 0.05), which could achieve the same therapeutic effect as ursodeoxycholic acid capsules. Conclusion: Dahuang Lingxian Prescription can reduce the secretion of “lithogenic bile” by inhibiting the inflammatory reaction and fibrosis of biliary system. Dahuang Lingxian Prescription has certain advantages in the treatment of gallbladder distention.

Gallbladder Distention, Dahuang Lingxian Prescription, Cell Ultrastructure, Signal Transduction, Traditional Chinese Medicine

Huang, X. , He, X. , Gan, Y. , Wang, B. , Zhang, M. , Dai, J. and Yu, Y. (2022) Experimental Study on the Treatment of Gallbladder Distention by Dahuang Lingxian Prescription. Journal of Biosciences and Medicines, 10, 82-92. doi: 10.4236/jbm.2022.1011007.