Author(s)

Daniel A. Abaye^1*, Emmanuel B. Odoom², Ernest Y. Boateng¹, Irene A. Agbo¹, John-Bosco Diekuu², Samuel Agana²

¹Department of Basic Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Volta Region, Ghana.
²ICT Directorate, University of Health and Allied Sciences, Ho, Volta Region, Ghana.

Clinical assessment of fluid volume status in children during malaria can be taxing and often inaccurate. During malaria, changes in fluid volume are rather multifarious and estimating this parameter, especially in sick children is very challenging for clinicians who frequently rely on indices such as long capillary refill times, tachycardia, central venous pressure and decreased urine volume as guides. Here, we present the UHAS-MIDA, an open-source software tool that calculates the red blood cell (RBC) concentration and blood volume during malaria in children determined using a stable isotope of chromium (⁵³Cr as the label) by gas chromatography-mass spectrometry in selective ion monitoring (GC/MS-SIM) analysis. A key component involves the determination of the compositions of the most abundant naturally occurring isotopes of Cr (⁵⁰Cr, ⁵²Cr, ⁵³Cr), and converting the proportions into a 3 × 3 matrix. To estimate unknown proportions of chromium isotopic mixtures from the measured abundances of three ions, an inverse matrix was calculated. The inverse together with several inputs is then used to calculate the corrected MS ion abundances. Thus, we constructed the software tool UHAS- MIDA using HTML, CSS/Bootstrap, JavaScript, and PHP scripting languages. The tool enables the user to efficiently determine RBC concentration and fluid volume. The source code, binary packages and associated materials for UHAS-MIDA are freely available at
https://github.com/bentil078/Abaye-et-al_UHASmida.

Mass Isotopomer Distribution Analysis, Inverse Matrix, GC/MS, Chromium Stable Isotopes, Blood Volume, Red Blood Cell Mass

Abaye, D. , Odoom, E. , Boateng, E. , Agbo, I. , Diekuu, J. and Agana, S. (2022) UHAS-MIDA Software Package: Mass Isotopomer Distribution Analysis-Applying the Inverse Matrix for the Determination of Stable Isotopes of Chromium Distribution during Red Blood Cell Labelling. Journal of Data Analysis and Information Processing, 10, 142-154. doi: 10.4236/jdaip.2022.102009.