Author(s)

Kaori Yama^*, Yoshiki Hasebe, Ayuka Inomata, Jun Miura, Ainari Konda

Pharmacotherapy, Hokkaido University of Science, Sapporo, Japan.

An increase in oxidative stress plays a key role in neurotoxicity induction and cell death, which leads to neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease. Cyanidin-3-glucoside (C3G) is a common anthocyanin and shows antioxidant activity in neuronal cells. Silent information regulator 2-related protein 1 (Sirt1) regulates antioxidant and anti-inflammatory effects. However, the effects of C3G on Sirt1 in neuronal cells remain unclear. This study evaluated the effect of C3G on Sirt1 expression and activity in human neuroblastoma (SH-SY5Y) cells. In the study, C3G increased the expression of Sirt1 and Sirt1 activity in SH-SY5Y cells. Additionally, C3G increased the expression of nuclear factor erythroid 2-related factor 2, a vital transcription factor for regulating the expression of antioxidant genes, as well as antioxidant enzymes such as superoxide dismutase and catalase. Moreover, C3G protected SH-SY5Y cells from oxidative stress. These results suggest that C3G decreased oxidative stress-induced cell injury by increasing the expression of Sirt1 and other antioxidant factors. Therefore, C3G might merit further investigation for use in attenuating the progress of neurodegenerative diseases.

Cyanidin-3-O-Glucocide, Sirtuin1, Anti-Senescence, Antioxidant, Oxidative Stress, Nerve Cells

Yama, K. , Hasebe, Y. , Inomata, A. , Miura, J. and Konda, A. (2022) Induction of Sirtuin1 Activity in SH-SY5Y Cells by Cyanidin-3-O-Glucocide Induced. Pharmacology & Pharmacy, 13, 35-48. doi: 10.4236/pp.2022.132003.