Introduction: Hypertension, often associated with hypervolemia, is common in chronic hemodialysis patients. However, the relationship between interdialytic weight gain (IDWG) and blood pressure (BP) is incompletely characterized. Our study seeks to appreciate the relationship between IDWG and BP in a cohort of chronic hemodialysis patients. Patients and Methods: It is a prospective study involving 52 chronic hemodialysis patients and 528 dialysis sessions following a hemodialysis program of 3 times a week, where BP and IDWG were measured before the session. The dialysis sessions occurred over a period of 30 days from January 02 to 30, 2019 in the Hemodialysis unit of Mohammed V Military Training Hospital. Demographic data and dialytic parameters were collected over a 30-day period then analyzed using the following statistical tools: correlation coefficient r, simple linear regression (univariate analysis) and multiple linear regression (multivariate analysis). Results: Mean age was 55 ± 12 years, 48% were men, 32.7% were diabetic and 61.5% were on antihypertensive therapy. In univariate analysis, each 1 kg increase in IDWG was associated with an increase of 3.3 mmHg in systolic BP (p = 0.01) and 2.7 mmHg in diastolic BP (p = 0.006). In multivariate analysis, after adjusting for confounding factors, an increase of 1 kg of IDWG was associated with an increase of 1.7 mmHg of systolic BP (p = 0.05) and 1.8 mmHg of diastolic BP (p = 0.05). Conclusion: Our results show that there is a significant association between IDWG and BP, however, its modest magnitude can’t explain alone the variations of BP in the chronic hemodialysis patients.