The polygraph-based Concealed Information Test (CIT) uses psychophysiological measures to determine whether there are crime-related information in suspects’ memory. This study explores the physiological indicators used in polygraph-based CIT and their effectiveness. Furthermore, we introduce the theoretical basis of physiological-based CIT. It is pointed out that further researches should thoroughly explore the different physiological responses and psychological process in the CIT paradigm and strengthen the comprehensive research on different psychological indicators.