Author(s)

Chenghu Liu^1,2*, Li Hou^1,2, Lili Liu^1,2, Zhonghua Qu³, Xin Wang^1,2, Xiaoxia Sun^1,2, Ping Wu^1,2, Yanping Shi^1,2

¹Shandong Quality Inspection Center for Medical Devices, Jinan, China.
²Shandong Key Laboratory of Biological Evaluation for Medical Devices, Jinan, China.
³Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Jinan, China.

In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC₅₀ values among different dilutions of AgNO₃ solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC₅₀ values of AgNO₅₀ solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC₅₀ value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.

Cytotoxicity, Silver Zirconium Phosphate, IC₅₀

Liu, C. , Hou, L. , Liu, L. , Qu, Z. , Wang, X. , Sun, X. , Wu, P. and Shi, Y. (2016) In Vitro Cytotoxicity Evaluation of Highly Absorbent Foam Dressings Based on Silver Zirconium Phosphate via IC₅₀ Value. Journal of Biomaterials and Nanobiotechnology, 7, 37-44. doi: 10.4236/jbnb.2016.71005.