Chemotherapeutic Effect of Withaferin A in Human Oral Cancer Cells - Journal of Cancer Therapy

JCT > Vol.6 No.8, August 2015

Chemotherapeutic Effect of Withaferin A in Human Oral Cancer Cells ()

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DOI: 10.4236/jct.2015.68080 3,543 Downloads 5,091 Views Citations

Author(s)

In-Hyoung Yang, Lee-Han Kim, Ji-Ae Shin, Sung-Dae Cho^*

Affiliation(s)

Department of Oral Pathology, School of Dentistry, Institute of Biodegradable Material, Institute of Oral Bioscience, Chonbuk National University, Jeonju, Republic of Korea.

ABSTRACT

Withaferin A (WA) is a bioactive compound derived from a medicinal plant Withania somnifera and has potential therapeutic effects against various types of cancers. The purpose of this study is to investigate an apoptotic effect of WA and identify its molecular target in HSC-3 and HSC-4 human oral cancer cell lines using Trypan blue exclusion assay, DAPI staining and western blotting. WA inhibited cell viability and induced apoptosis in a concentration- or time-dependent manner, as evidenced by induction of nuclear condensation and fragmentation, activation of caspase 3 and poly (ADP-ribose) polymerase (PARP) cleavage. WA-induced apoptosis was partly diminished by Z-VAD, a pancaspase inhibitor. WA also increased Bim and Bax protein in HSC-3 and HSC-4 cells, respectively. These results suggest that WA may be a potential chemotherapeutic drug candidate against human oral cancer.

KEYWORDS

Oral Cancer, Withaferin A, Apoptosis, Bim, Bax

Share and Cite:

Yang, I. , Kim, L. , Shin, J. and Cho, S. (2015) Chemotherapeutic Effect of Withaferin A in Human Oral Cancer Cells. Journal of Cancer Therapy, 6, 735-742. doi: 10.4236/jct.2015.68080.

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