Author(s)

Kouadio Amani¹, Lassina Fondio², Konate Ibrahim^1*, Mako François D. P. N’Gbesso², Beugre G. A. Maxwell¹, Tidou Abiba Sanogo¹, Abdelkarim Filali-Maltouf³

¹UFR Agro-Forestry and Environment, Department of Biochemistry and Microbiology, Laboratory of Agrovalorization, Jean Lorougnon Guede University, Daloa, Côte d’Ivoire.
²National Center for Agronomic Research (CNRA), Bouaké, Côte d’Ivoire.
³Faculty of Sciences, Laboratory of Microbiology and Molecular Biology, University Mohammed V-Agdal, Rabat, Morocco.

Soybean [Glycine max (L.) Merrill] is an important crop known to improve population nutritional status and increase soil fertility and its productivity through biological nitrogen fixation. In Côte d’Ivoire, the introduced Bradyrhizobium japonicum used as inoculum had slight compatibility to several soybean varieties compromising their vulgarization. Therefore, the present study was conducted to examine the infectiveness and the effectiveness of indigenous rhizobial isolates on three soybean varieties (Canarana, Doko and Piramana) cultivated in Côte d’Ivoire. The experiment was conducted with potted plant filled with sterilized sand and was statistically laid in Completely Randomized Design (CRD) with sixteen (16) natives rhizobia, one (01) reference strain and uninoculated control (with or without nitrogen) with three replications. The results showed that inoculation significantly improved nodule number, nodule dry weight, plant height and total dry matter of soybean over the negative control treatment. Among the inoculated treatments, five indigenous rhizobia RSC119, RSC324, RSC502, RSC504 and RSC508 significantly (P < 0.05) increased the nodulation and plant growth parameters than B. japonicum (IRAT FA3) with Doko and Piramana. RSC502 produced highest nodule number (64) on Piramama, nodule dry weight was most promoted with RSC504 (321 mg ·plant^-1) compared to IRAT FA3 strain (95 mg·plant^-1) on Doko. The higher effectiveness was recorded with RSC119, RSC504 and RSC502 with 206.73%; 201.79% and 200.45% respectively compared to TN (100%). The correlation analysis indicated significant association of nodule number and total biomass indicating the importance of symbiotic nitrogen fixation. Based on their infectiveness and their effectiveness, the isolates RSC119, RSC324, RSC502, RSC504 and RSC508 could be used as elite local rhizobia and tested in field conditions to establish their potential contribution on soybean productivity.

Selection, Indigenous Rhizobia, Inoculation, Soybean, Côte d’Ivoire

Amani, K. , Fondio, L. , Ibrahim, K. , D. P. N’Gbesso, M. , A. Maxwell, B. , Abiba Sanogo, T. and Filali-Maltouf, A. (2020) Response of Indigenous Rhizobia to the Inoculation of Soybean [Glycine max (L.) Merrill] Varieties Cultivated under Controlled Conditions in Côte d’Ivoire. Advances in Microbiology, 10, 110-122. doi: 10.4236/aim.2020.103010.