Author(s)

Lucien Murhula Namegabe^1*, Serigne Omar Sarr^2,3, Yérim Mbagnick Diop^2,3

¹Department of Galenic Pharmacy, Official University of Bukavu, Bukavu, Democratic Republic of the Congo.
²Analytical Chemistry and Food Sciences Laboratory, Cheikh Anta Diop University, Dakar, Senegal.
³National Medecines Control Laboratory, Dakar, Senegal.

The purpose of this study is to develop and validate a method for the analysis of tetracycline capsules by spectrofluorimetry. A pH 9 borate buffer was used as diluent of tetracycline after reaction with magnesium salt at the excitation wavelength of 372 nm and 516 nm of emission. A linear response was observed between 0.25 μg/mL and 1.5 μg/mL with a correlation coefficient (R) of 0.9998. The detection and quantification limits found are 0.0125 μg/mL and 0.0412 μg/mL respectively. The proposed method proved trueness with a recovery between 99.88% and 101.10%. The relative standard deviations of repeatability and intermediate precision found ≤2.88% reflected a good precision of the method. The proposed method is therefore valid within the limits of 90% to 110%. The proposed method was applied to the quality control of 9 tetracycline samples from market and gave results in accordance with the pharmacopoeia standards.

Validation, Spectrofluorimetry, Tetracycline

Namegabe, L. , Sarr, S. and Diop, Y. (2018) Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules. American Journal of Analytical Chemistry, 9, 162-170. doi: 10.4236/ajac.2018.93014.