Establishment and Application of Digital RT-PCR Assay for Detection of Avian Influenza Virus H9 Subtype - Advances in Microbiology

AiM > Vol.7 No.11, November 2017

Establishment and Application of Digital RT-PCR Assay for Detection of Avian Influenza Virus H9 Subtype ()

HTML XML

Download as PDF (Size: 1850KB) PP. 760-768

DOI: 10.4236/aim.2017.711061 1,357 Downloads 2,539 Views

Author(s)

Bin Wu^1*, Lin Zhang¹, Liming Su², Huijun Zhao¹, Xiaoping Cai¹

Affiliation(s)

¹Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, China.
²China Certification & Inspection Group Liaoning Co. Ltd., Dalian, China.

ABSTRACT

A digital RT-PCR method for rapid detection of H9 subtype influenza was established by comparing the two methods of digital RT-PCR and real-time quantitative RT-PCR. The sensitivity, specificity and reproducibility of the two methods for H9 were determined by gradient dilution using the same pair of primers and probes. Both methods were able to detect 10⁴ times diluted H9 pathogens, while digital RT-PCR could detect H9 in single droplets, and its sensitivity was higher than real-time quantitative RT-PCR. At the same time, the specificities of both methods were very strong, with no amplification reactions for H3N2, H4N2, H6N2. The reproducibility of the two methods were also good. Digital RT-PCR has a higher sensitivity than real-time quantitative RT-PCR and could play an important role in the rapid detection of H9 subtype influenza virus.

KEYWORDS

Avian Influenza Virus H9 Subtype (H9), Digital RT-PCR, Real-Time Quantitative RT-PCR, Sensitivity, Specificity

Share and Cite:

Wu, B. , Zhang, L. , Su, L. , Zhao, H. and Cai, X. (2017) Establishment and Application of Digital RT-PCR Assay for Detection of Avian Influenza Virus H9 Subtype. Advances in Microbiology, 7, 760-768. doi: 10.4236/aim.2017.711061.

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies