The paper elaborates the features and superiority of applying multimedia technology to aquatic organisms in a Science and Life Technology course. The augmented reality application of multimedia technology in aquatic organisms’ instruction has its own features, teaching information capacity, interactive user interface. The paper concludes the superiority of Unity and Vuforia AR instruction from five perspectives; meanwhile, the paper elaborates its disadvantages. An AR-based instruction attitude questionnaire was conducted at the end of the experiment to obtain learners’ perspectives of the system. Learners in AR approach demonstrate higher motivation and concentrate their attention on the learning tasks. Learners showed more positive attitude in willingness to use the AR-based instruction to improve learning interests. This study provided insights for better understanding the design, theory and practice of e-learning through augmented reality technology.