Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol - Journal of Biomaterials and Nanobiotechnology

JBNB > Vol.4 No.3, July 2013

Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol ()

HTML XML

Download as PDF (Size: 688KB) PP. 242-246

DOI: 10.4236/jbnb.2013.43030 3,091 Downloads 4,595 Views Citations

Author(s)

Nikita O. Ronzhin, Alexey V. Baron, Ekaterina S. Mamaeva, Alexey P. Puzyr, Vladimir S. Bondar

Affiliation(s)

Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia..

ABSTRACT

Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of enzymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes exhibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling (Н₂О₂—4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and various buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional activity after long storage at + 4°C.

KEYWORDS

Nanodiamond; Test-System; Glucose; Cholesterol

Share and Cite:

N. Ronzhin, A. Baron, E. Mamaeva, A. Puzyr and V. Bondar, "Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 3, 2013, pp. 242-246. doi: 10.4236/jbnb.2013.43030.

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies