In order to achieve the five degree of freedom manipulator motion simulation based on virtual reality environments, the calculation of the manipulator motion path of the joint node, established the mathematical models and three dimensional models, completed five degree of freedom manipulator real-time interactive simulation study based on the EON Studio platform, finished the experimental simulation of mechanical arm motion path. Provide some theoretical basis for the multiple degrees of freedom, multi-channel input and multiple perception mechanical movement of virtual interactive simulation-based research in the EON environment.