Dr. Steven N. Fiering

Dartmouth Medical School, USA

Email: Fiering@Dartmouth.edu

Qualifications

1974 B.Sc., University of Michigan

Publications (Selected)

1. Hug, B. A., R. L. Wesselschmidt, S. Fiering, M. A. Bender, E. Epner, M. Groudine, T.J. Ley; Analysis of Mice Containing a Targeted Deletion of ß-globin Locus Control Region 5’ Hypersensitive Site 3; Molecular and Cellular Biology, 16, 2906-2912, 1996.
2. Walters, M., W. Magis, S. Fiering, J. Eidemiller, D. Scalzo, M. Groudine; Transcriptional Enhancers Act to Suppress Position Effect Variegation in Cis; Genes and Development, 10, 185-196, 1996.
3. Diecken, E., E.Epner, S. Fiering, K. Fournier, M. Groudine; Efficient Gene Targeting of the Human ß-globin in Recombination-Proficient Chicken/Human Microcell Hybrids; Nature Genetics, 12, 174-182, 1996.
4. Magis, W., S. Fiering, M. Groudine, D. I. K. Martin; An Upstream Activator of transcription Coordinately Increases the Level and Epigenetic Stability of Gene Expression; Proceedings of the National Academy of Sciences USA, 93: 13914-13918, 1996.
5. Zambrowicz, B. P., A. Imamoto, S. Fiering, L. A. Herzenberg, W. G. Kerr, P. Soriano; Disruption of Overlapping Transcripts in the ROSA ßgeo 26 Gene Trap Strain Leads to Widespread Expression of ß-galactosidase in Mouse Embryos and Hematopoietic Cells; Proceedings of the National Academy of Sciences USA, 94: 3789-3794, 1997.
6. Garrick, D., S. Fiering, D. I. K. Martin, E. Whitelaw; Cremediated Reduction of Copies within a Transgene Array Provides Evidence for Repeat-induced Gene Silencing in Mammals; Nature Genetics 18:56-59, 1998.
7. Seibler, J., D. Schubeler, S. Fiering, M. Groudine, J. Bode; DNA Cassette Exchange in ES cells Mediated by FLP Recombinase: An Efficient Strategy for Repeated Modification of Tagged Loci by Marker-free Constructs; Biochemistry, 37:6229-6234, 1998.
8. Ley, T. J., B. Hug, S. Fiering, E. Epner,. M. A. Bender, M. Groudine; Reduced beta-globin gene expression in adult mice containing deletions of locus control region 5’ HS-2 or 5’ HS-3; Annals of the New York Academy of Sciences, 850:45-53, 1998.
9. Epner, E, A. Reik, D. Cimbora, A. Telling, M. A. Bender, S. Fiering, T. Enver, D. I. K. Martin, M. Kennedy, G. Keller, M. Groudine; The ß-globin LCR is not necessary for an open chromatin structure or developmentally regulated transcription of the native mouse ß-globin locus; Molecular Cell 2:447-455, 1998.
10. Bender, M. A., A. Reik, J. Close, A. Telling, E. Epner, S. Fiering, R. Hardison, M. Groudine; Description and targeted deletion of 5’ hypersensitive site 5 and 6 of the mouse ß-globin locus control region; Blood, 92:4394-4403, 1998.
11. Howell, AL, T. N. Thacker, F. Li, S. Fiering, R. F. Graziano, J. Goldstein, and M. W. Fanger. Targeting HIV-1 gp120 to the high affinity Fc receptor (FcgRI, CD64) on myeloid antigen presenting cells: implications for enhancing vaccine responses. Current Topics in Virology 1:62-70, 1999.
12. Feng Y-Q, J. Seibler, R. Alami, A. Eisen, K. A. Westerman, P. Leboulch, S. Fiering, E. Bouhassira; Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange. Journal of Molecular Biology, 292: 779-785, 1999.
13. Alami, R., J. M. Greally, K. Tanimoto, S. Hwang, Y.Q. Feng, J.D. Engel, S. Fiering, E. Bouhassira; The ß-globin LCR controls expression levels but does not block heterocellular position effects. Human Molecular Genetics 9:631-6, 2000.
14. Alami, R., M. A. Bender, Y-Q. Feng, T. J. Ley, S. Fiering, M. Groudine, EE Bouhassira; Deletions within the mouse ß-globin LCR preferentially reduce ß-minor expression. Genomics 63:417-424, 2000.
15. Bender, M. A., M.G. Mehaffey, A. Telling, B. Hug, T.J. Ley, M. Groudine, S. Fiering; Independent formation of DNaseI hypersensitive sites in the murine ß-globin locus control region. Blood, 95:3600-4, 2000.
16. Feng, YQ, M. Lorincz, S. Fiering, J.M. Greally, E.E. Bouhassira;Position effects are influenced by the orientation of a transgene with respect to flanking chromatin. Molecular and Cellular Biology, 21, 298-309, 2001.
17. Bender, MA, J. N. Roach, J. Halow, J. Close, R. Alami, E. E. Bouhassira, M. Groudine, S. Fiering; Targeted deletion of 5’HS1 and 5’HS4 of the ß-globin LCR reveals additive activity of DnaseI hypersensitive sites, Blood (plenary paper), 98, 2022-7, 2001
18. Schneider, MJ, S. Fiering, S. E. Pallud, A.E. Parlow, D. L. St. Germain, V. A. Galton; Targeted disruption of the type 2 selenodeiodinase gene (DIO2) results in phenotype of pituitary resistance to thyroxine, Molecular Endocrinology, 15, 2137-48, 2001.
19. Eszterhas, S, D. I. K. Martin, E.E. Bouhassira, S. Fiering; Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position, Molecular and Cellular Biology 22:469-79, 2002.
20. Krieser, R.J., KS. MacLea, DS Longnecker, JL Fields, S. Fiering and A. Eastman. Deoxyribonuclease IIa is required for DNA digestion during the phagocytic phase of apoptosis and its loss causes perinatal lethalality, Cell Death Different., 9: 956-962, 2002.
21. Hernandez, A., S. Fiering, E. Martinez, V.A. Galton, D. St.Germain;The gene locus encoding idothyroxine deiodinase type 3 (Dio3) is imprinted in the fetus and expresses antisense transcripts. Endocrinology, 143:4483-6, 2002.
22. Hu, X., M. Bulger, J.N. Roach, S.K. Eszterhas, E. Olivier, E.E. Bouhassira, M. Groudine, S. Fiering; Promoters of the murine embryonic ß-like globin genes Ey and ßh1 do not compete for interaction with the ß-globin locus control region. Proceedings of the National Academy of Sciences USA 100:1111-5, 2003.
23. Sutter, N.B., D. Scalzo, S. Fiering, M. Groudine, D. I. K. Martin; Chromatin insulation by a transcriptional activator. Proceedings of the National Academy of Sciences USA 100:1105-10, 2003.
24. Rosenbauer, F., K. Wagner, J.L. Kutok, H. Iwasaki, M. LeBeau, Y. Okuno, K. Akashi, S. Fiering, D.G. Tenen, Acute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU. 1. Nature Genetics, 36: 624-30, 2004.
25. Kato, KC, S. Martinez, P. Russel, Nurden, A. Nurden, S. Fiering, J. Ware; Genetic deletion of mouse platelet glycoprotein Ibbeta produces a Bernard-Soulier phenotype with increased alpha-granule size. Blood, 104: 2339-44, 2004.
26. Kang, HC, JH Chae, YH Lee, M-A Park,JH Shin, S-H Kim, S-K Ye, YS Cho, S Fiering, CG Kim; Erythroid cell specific a-globin gene regulation by the CP2 transcription factor family. Molecular and Cellular Biology, 25:6005-20, 2005.
27. Schneider, M., S. Fiering, B. Thai, S-Y Wu, E St. Germain, AF Parlow, DL St. Germain, VA Galton; Targeted disruption of the type 1 selenodeiodinase gene (Dio1) results in marked changes in thyroid hormone economy in mice. Endocrinology, 147:580-9, 2006.
28. Hernandez, A, ME Martinez, S Fiering, VA Galton, D St. Germain: Type 3 deiodinase is critical for the maturation and function of the thyroid axis. Journal of Clinical Investigation, 116:476-84, 2006.
29. Hu, X, M. Bulger, M. A. Bender, J. Fields, M. Groudine, S. Fiering: Deletion of the core region of 5’HS2 of the mouse ß-globin locus control region reveals a distinct effect in comparison with human ß-globin transgenes. Blood, 107:821-6, 2006.
30. Hu, X, Eszterhas, S, Pallazzi, N, Bouhassira, EE, Fields, J, Tanabe, O, Gerber, SA. Bulger, M, Engel JD, Groudine, M, Fiering, S: Transcriptional Interference among the murine-like globin genes. Blood 109:2210-2216, 2007.
31. Barre, L, C. Richardson, M. F. Hirshman, J. Brozinick, S. Fiering, B. E. Kemp, L. J. Goodyear,L.A. Witters A genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation.American Journal of Physiology: Endocrinology and Metabolism 292: E802-E811, 2007.
32. Ma, Y, S. Fiering, C. Black, X. Liu, Z. Yuan, V. A. Memoli, D. J. Robbins, H. A. Bentley, G. J. Tsongalos, E. Dimidenko, S. J. Freemantle, E. Dmitrovsky. Transgenic Cyclin E triggers dysplasia and multiple pulmonary adenocarcinomas. Proceedings of the National Academy of Sciences USA, 104:4089-94, 2007.
33. Hsu, Y. M., R. Mabaera, C. H. Lowrey, D. I. K. Martin, S. Fiering. CpG hypomethylation inlarge domain encompassing the embryonic eta-like globin genes in primitive erythrocytes. Molecular and Cellular Biology 27:5047-54, 2007.
34. Srinivasulu, S., S. A. Acharya, M. Prabhakaran, M.E. Fabry, R. Alami, S. Fiering, E. E. Bouhassira, R.L. NagelHbS-Savaria: The antipolymerization effect of a single mutation in human alpha-chains. Protein Journal. 26: 523-32, 2007.
35. Jain, S, Vinukonda, G, Fiering, SN, Kumar, A: A halotype of human angiotensinogen gene containing-217A increases blood pressure in transgenic mice as compared to 217G. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 295(6):R1849-57, 2008.
36. Hsu, M, C. A. Richardson, E. Olivier, C. Qui, E. E. Bouhassira, C. H. Lowrey, S. Fiering: Complex developmental patterns of histone modifications associated with the human eta-globin switch in primary cells. Experimental Hematology. 37:799-806, 2009.
37. Coon, C. I., S. Fiering, J. Gaudet, C.A. Wyatt, C. E. Brinckerhoff: Site controlled transgenic mice validating increased expression from human matrix metalloproteinase (MMP-1) promoter due to a naturally occurring SNP, Matrix Biology 28:425-31 (2009).
38. Fromm, G., C. de Vries, R. Byron, J. Fields, S. Fiering, M. Groudine, M. A. Bender, J. Palis, and M. Bulger: Histone hyperacetylation within the beta-globin locus is context-dependent and precedes high-level gene expression. Blood, 114:3479-88 (2009).
39. Guan, Z., C. M. Hughes, S. Kosiyatrukul, P. Norio, R. Sen, S. Fiering, C. D. Allis, E. E, Bouhassira, C. L. Schildkraut. Decreased replication origin activity in temporal transition regions. Journal of Cell Biology 187:623-35 (2009).
40. Liu, X, L. F. Sempere, F. Galimberti, S.J. Freemantle, C. Black, K. H. Dragnev, Y. Ma, S. Fiering, V. Memoli. J. Li, S. Kauppinen E. Dmitrovsky: Uncovering Growth-Suppressive MicroRNAs in Lung Cancer. Clinical Cancer Research 15: 1177-83, (2009).
41. Crane-Godreau, M, M.M. Maccani, S.K.Eszterhas, S.L. Warner, J.A. Jukosky, S. Fiering: Exposure to cigarette smoke disrupts CCL20-mediated antimicrobial activity in respiratory epithelial cells. Open Immunology Journal 2: 86-93, (2010).
42. Lathrop, MJ, L Chakrabarti, J Eng, CH Rhodes, T. Lutz, A. Nieto, HD. Liggitt, S Warner, J. Fields, R. Stoger, S. Fiering: Deletion of the Chd6 exon 12 affects motor coordination. Mammalian Genome 3-4: 130-42 (2010).
43. Akada, H, D. Yan, H. Zou, S. Fiering, RE Hutchinson, MG Mohi:Conditional expression of heterozygous or homozygous Jak2V617F from its endogenous promoter induces a polycythemia vera-like disease. Blood, 115: 3589-97, (2010).
44. Berland, R., S. Fiering, H. Wortis: A conserved enhancer element differentially regulates developmental expression of CD5 in B and T cells. Journal of Immunology 185:7537-43, (2010).
45. Jain, S., A. Tillinger, B. Mopidevi, VG Pandey, C Chauhan, S Fiering, S Warming, A. Kumar: Transgenic mice with -6A haplotype of the human angiotensinogen gene have increased blood pressure compared to -6G haplotype, Journal Biol. Chem, 285:41172-86, 2010.
46. Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M.; An embryonic stage-specific enhancer within the murine {beta}-globin locus mediates domain-wide histone hyperacetylation. Blood 117; 52-7-14 2011.
47. Cadiz-Rivera, Brenda & Fromm, George & Vries, Christina & Fields, Jennifer & Mcgrath, Kathleen & Fiering, Steven & Bulger, Michael. (2014). The Chromatin “Landscape” of a Murine Adult β-Globin Gene Is Unaffected by Deletion of Either the Gene Promoter or a Downstream Enhancer. PloS one. 9. e92947. 10.1371/journal.pone.0092947.
48. Toraya-Brown, Seiko & Sheen, Mee & Zhang, Peisheng & Chen, Lei & Baird, Jason & Demidenko, Eugene & Turk, Mary & Hoopes, P. & Conejo-Garcia, Jose & Fiering, Steven. (2014). Local hyperthermia treatment of tumors induces CD8+ T cell-mediated resistance against distal and secondary tumors. Nanomedicine: Nanotechnology, Biology and Medicine. 10. 10.1016/j.nano.2014.01.011.
49. Sheen, Mee & Lizotte, Patrick & Toraya‐Brown, Seiko & Fiering, Steven. (2014). Stimulating antitumor immunity with nanoparticles. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 6. 10.1002/wnan.1274.
50. Fiering, Steven & Knutson, Keith. (2015). Cancer Vaccines in the new generation of immunotherapies. Cancer Vaccines. 1. 10.1515/canv-2015-0001.
51. Ndong, Christian & Toraya-Brown, Seiko & Kekalo, Katsiaryna & Baker, Ian & Gerngross, Tillman & Fiering, Steven & Griswold, Karl. (2015). Antibody-mediated targeting of iron oxide nanoparticles to the folate receptor alpha increases tumor cell association in vitro and in vivo. International Journal of Nanomedicine. 10. 2595-617. 10.2147/IJN.S79367.
52. Zhao, Hongliang & Verma, Deeptak & Li, Wen & Choi, Yoonjoo & Ndong, Christian & Fiering, Steven & Bailey-Kellogg, Chris & Griswold, Karl. (2015). Depletion of T Cell Epitopes in Lysostaphin Mitigates Anti-Drug Antibody Response and Enhances Antibacterial Efficacy In Vivo. Chemistry & biology. 22. 629-639. 10.1016/j.chembiol.2015.04.017.
53. Lee, Karin & Twyman, Richard & Fiering, Steven & Steinmetz, Nicole. (2016). Virus‐based nanoparticles as platform technologies for modern vaccines. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 8. 10.1002/wnan.1383.
54. Varn, Frederick & Mullins, David & Arias-Pulido, Hugo & Fiering, Steven & Cheng, Chao. (2016). Adaptive immunity programs in breast cancer. Immunology. 150. 10.1111/imm.12664.
55. Sheen, M & Marotti, J & Allegrezza, Michael & Rutkowski, Melanie & Conejo-Garcia, J & Fiering, Steven. (2016). Constitutively activated PI3K accelerates tumor initiation and modifies histopathology of breast cancer. Oncogenesis. 5. e267. 10.1038/oncsis.2016.65.
56. Lizotte PH, Wen AM, Sheen MR, Fields J, Rojanasopondist P, Steinmetz NF, Fiering S. In situ vaccination with cowpea mosaic virus nanoparticles suppresses metastatic cancer. Nat Nanotechnol. 2016; 11:295-303. DOI: 10.1038/NNANO.2015.292
57. Varn, Frederick & Wang, Yue & Mullins, David & Fiering, Steven & Cheng, Chao. (2017). Systematic Pan-Cancer Analysis Reveals Immune Cell Interactions in the Tumor Microenvironment. Cancer Research. 77. canres.2490.2016. 10.1158/0008-5472.CAN-16-2490.
58. Lathrop, Melissa & Rhodes, C. & Fiering, Steven. (2017). CHD6 (chromodomain helicase DNA binding protein 6). Atlas of Genetics and Cytogenetics in Oncology and Haematology. 10.4267/2042/62143.
59. Hoopes P, Wagner R, Duval K, Kang K, Gladstone D, Moodie K, Crary-Burney M, Arias-Pulido H, Veliz F, Steinmetz N, Fiering S. Treatment of canine oral melanoma with nanotechnology-based immunotherapy and radiation. Mol Pharmaceutics 2018; Apr12 doi: 10.1021/acs.molpharmaceut.8b00126. PMID:2961380.
60. Sheen MR, Fiering S.  In situ vaccination: Harvesting low-hanging fruit on the cancer immunotherapy tree.Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2018 Apr 18:e1524. doi: 10.1002/wnan.1524. Review. PMID: 29667346
61. Duval, Kayla & Vernice, Nicholas & Wagner, Robert & Fiering, Steven & Petryk, James & Lowry, Gabriela & Tau, Steven & Yin, John & Houde, Georgia & Chaudhry, Aneeq & Hoopes, P.. (2019). Immunogenetic effects of low dose (CEM43 30) magnetic nanoparticle hyperthermia and radiation in melanoma cells. International Journal of Hyperthermia. 36. 37-46. 10.1080/02656736.2019.1627433.
62. Fiering, Steven & Evans, Sharon. (2019). Introduction to thermal therapy and immunotherapy: at the crossroads of new discovery. International Journal of Hyperthermia. 36. 1-2. 10.1080/02656736.2019.1659427.
63. Sethuraman, Sri & Singh, Mohit & Patil, Girish & Li, Shitao & Fiering, Steven & Hoopes, P. & Guha, Chandan & Malayer, Jerry & Ranjan, Ashish. (2020). Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity. Theranostics. 10. 3397-3412. 10.7150/thno.42243.
64. Shahgolzari, Mehdi & Pazhouhandeh, Maghsoud & Milani, Morteza & Yari, Ahmad & Fiering, Steven. (2020). Plant viral nanoparticles for packaging and in vivo delivery of bioactive cargos. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 12. e1629. 10.1002/wnan.1629.
65. Giddings, Emily & Champagne, Devin & Wu, Meng-Han & Laffin, Joshua & Thornton, Tina & Valenca Pereira, Felipe & Culp-Hill, Rachel & Fortner, Karen & Romero, Natalia & East, James & Cao, Phoebe & Arias-Pulido, Hugo & Sidhu, Karatatiwant & Silverstrim, Brian & Kam, Yoonseok & Kelley, Shana & Pereira, Mark & Bates, Susan & Bunn, Janice & Rincon, Mercedes. (2021). Mitochondrial ATP fuels ABC transporter-mediated drug efflux in cancer chemoresistance. Nature Communications. 12. 2804. 10.1038/s41467-021-23071-6.
66. Alonso-Miguel, Daniel & Valdivia, Guillermo & Guerrera, Diego & Pérez-Alenza, Maria & Pantelyushin, Stanislav & Alonso, Angela & Beiss, Veronique & Fiering, Steven & Steinmetz, Nicole & Suarez Redondo, Maria & Berg, Johannes & Peña, Laura & Arias-Pulido, Hugo. (2022). Neoadjuvant in situ vaccination with cowpea mosaic virus as a novel therapy against canine inflammatory mammary cancer. Journal for ImmunoTherapy of Cancer. 10. e004044. 10.1136/jitc-2021-004044.

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