Prof. Pablo Iván Nikel

The Novo Nordisk Foundation Center for Biosustainability, Denmark

Email: pabnik@biosustain.dtu.dk

Qualifications

2009  Ph.D., University of San Martin, Molecular Biology & Biotechnology
2004  B.Sc., University of San Martin, Biotechnology

Publications (Selected)

1. Della Valle, S., Orsi, E., Creutzburg, S. C., Jansen, L. F., Pentari, E. N., Beisel, C. L., ... & Patinios, C. (2025). Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system. Trends in Biotechnology.
2. Orsi, E., Schulz-Mirbach, H., Cotton, C. A., Satanowski, A., Petri, H. M., Arnold, S. L., ... & Nikel, P. I. (2025). Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection. Nature Communications, 16(1), 2168.
3. Federici, F., Luppino, F., Aguilar-Vilar, C., Mazaraki, M. E., Petersen, L. B., Ahonen, L., & Nikel, P. I. (2025). CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative genome and pathway engineering of Gram-negative bacteria. Metabolic Engineering, 88, 180-195.
4. Bergen, D., Puiggené, Ò., Marcellin, E., Speight, R. E., Nikel, P. I., & Ebert, B. E. (2025). Co-substrate free valorisation of lignin monomers by assimilation of C1 and C2 by-products. bioRxiv, 2025-02.
5. Lammens, E. M., Volke, D. C., Kerremans, A., Aerts, Y., Boon, M., Nikel, P. I., & Lavigne, R. (2025). Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida. Communications Biology, 8(1), 1-15.
6. Timmis, K., Karahan, Z. C., Ramos, J. L., Koren, O., Pérez‐Cobas, A. E., Steward, K., ... & Häggblom, M. (2025). Microbes Saving Lives and Reducing Suffering. Microbial biotechnology, 18(1), e70068.
7. Kozaeva, E., Nieto-Domínguez, M., Tang, K. K. Y., Stammnitz, M., & Nikel, P. I. (2025). Leveraging engineered Pseudomonas putida minicells for bioconversion of organic acids into short-chain methyl ketones. ACS Synthetic Biology.
8. Bozkurt, E. U., Ørsted, E. C., Volke, D. C., & Nikel, P. I. (2025). Accelerating enzyme discovery and engineering with high-throughput screening. Natural Product Reports.
9. Orsi, E., Hernández-Sancho, J. M., Remeijer, M. S., Kruis, A. J., Volke, D. C., Claassens, N. J., ... & Nikel, P. I. (2024). Harnessing noncanonical redox cofactors to advance synthetic assimilation of one-carbon feedstocks. Current Opinion in Biotechnology, 90, 103195.
10. Favoino, G., Krink, N., Schwanemann, T., Wierckx, N., & Nikel, P. I. (2024). Enhanced biosynthesis of poly (3‐hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl‐CoA availability. Microbial Biotechnology, 17(11), e70044.
11. Hernández-Sancho, J. M., Boudigou, A., Alván-Vargas, M. V., Freund, D., Arnling Bååth, J., Westh, P., ... & Nikel, P. I. (2024). A versatile microbial platform as a tunable whole-cell chemical sensor. Nature Communications, 15(1), 8316.
12. de Lorenzo, V., Pérez-Pantoja, D., & Nikel, P. I. (2024). Pseudomonas putida KT2440: the long journey of a soil-dweller to become a synthetic biology chassis. Journal of bacteriology, 206(7), e00136-24.
13. Didion, Y. P., Vargas, M. V. G. A., Tjaslma, T. G., Woodley, J., Nikel, P. I., Malankowska, M., ... & Pinelo, M. (2024). A novel strategy for extraction of intracellular poly (3-hydroxybutyrate) from engineered Pseudomonas putida using deep eutectic solvents: Comparison with traditional biobased organic solvents. Separation and Purification Technology, 338, 126465.
14. Timmis, K., Hallsworth, J. E., McGenity, T. J., Armstrong, R., Colom, M. F., Karahan, Z. C., ... & Serna, J. G. (2024). A concept for international societally relevant microbiology education and microbiology knowledge promulgation in society. Microbial Biotechnology, 17(5), e14456.
15. Orsi, E., Schada von Borzyskowski, L., Noack, S., Nikel, P. I., & Lindner, S. N. (2024). Automated in vivo enzyme engineering accelerates biocatalyst optimization. Nature Communications, 15(1), 3447.
16. Krink, N., Nikel, P. I., & Beisel, C. L. (2024). A Hitchhiker’s guide to CRISPR editing tools in bacteria: CRISPR can help unlock the bacterial world, but technical and regulatory barriers persist. EMBO reports, 25(4), 1694-1699.
17. Meliawati, M., Volke, D. C., Nikel, P. I., & Schmid, J. (2024). Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production. Microbial Biotechnology, 17(3), e14438.

Profile Details

WoS ResearcherID: L-4146-2014

https://orcid.org/0000-0002-9313-7481

http://www.sem-cfb.com/pabloresume.html

https://orbit.dtu.dk/en/persons/pablo-ivan-nikel

https://scholar.google.com/citations?user=flmgvvwAAAAJ&hl=en

https://www.researchgate.net/profile/Pablo-Ivan-Nikel