Prof. David W. Barnes

School of Science and Technology

Georgia Gwinett College, USA

Email: dbarnes1@ggc.edu

Qualifications

1973-1977   Ph.D., Department of Microbiology, Vanderbilt University, USA
1967-1971   B.S., Department of Molecular Biology, Vanderbilt University, USA

Publications (Selected)

1. Li, F. An, H., Seymour, T. A. and Barnes, D. W., 2000, Rainbow trout cystatin C: expression in E. coli and properties of the recombinant protease inhibitor, Comparative Biochemistry and Physiology, 125, 493-502  
2. Barnes, D. W. and Sato, G. H., 2000, Cell culture systems, in Tissue Engineering, second edition, (P. Lazarrini, ed.) pp. 111-118. 
3. Sato, J. D., Barnes D. W et al., 2000, Specific cell types and their requirements, in Basic Cell Cultures (J.M. Davis, ed.) Oxford Univ. Press, pp 227-274. 
4. Slinskey, A., Barnes, D., Pipas, J.M., 2000, SV40 large T antigen J domain and RB binding motif are sufficient to block apoptosis induced by growth factor withdrawal in a neuronal stem cell line. Journal of Virology 73, 6791-6799  
5. Murayama, K., Singh, N. N., Helmrich, A. and Barnes, D. W., 2000, Neural cell lines, in Protocols for Neural Cell Cultures (eds., Federhoff, S. and Richards, A.), pp 219-228. 
6. Singh, N. N., Fischer, K. A., Hedstrom, O. and Barnes, D. W., 2000, Fibroblast growth factor suppresses neurogenesis in primary cultures of early zebrafish embryo cells, Journal of Marine Biotechnology, 3, 27-35. 
7. Buck, C., Helmrich, A., Mericko, P., Toumadje, A., Kusumoto, K., Walsh, C., Davis, C. and Barnes, D., 2001, Cell culture and retrovirus expression from a tumor of a moray eel, Marine Biotechnology, 3, 193-202. 
8. Lerner, J. P., Bixler, L. M., Ivanchenko, M., McCormick, R.S., Bender , R. C., Hahn,, U.E., Toumadje, A., Barnes, D., and Bayne, C.J., 2001, Axenic in vitro development and birth of daughter sporocysts of Schistosoma mansoni, Journal of Parasitology, 87, 1167-1168. 
9. Barnes, D., Collodi , P., and Shirihata, S., 2001, Genetic manipulation in fish and fish cell cultures, Handbook of Animal Cell Technology, Kluwer Press (ed., H. Murakami).  
10. Mericko, P., Sharps, A., Chen L., Kusamoto, K., Toumadje, A., Chapline, C., Sato, D. and Barnes D. W., 2001, Basic concepts and techniques of cell culture, Encyclopedia of Life Sciences, Nature Publishing Group.  
11. Kusumoto, K., Parton, A., Mericko, P., Chen, L., Sato, J.D., Shirahata, S., Tokumaru, S., and Barnes, D., 2001, The protective anti-oxidant effects of kefir on neural stem cells. Animal Cell Technology 12, 353-358.  
12. Sato, J.D., Barnes, D, Hayashi I, Hayashi J, Hoshi H, Kawamoto T, McKeehan W, Matsuda R, Matsuzaki K, Okamoto T, Serrero G, Sussman D and Kan M, 2002, Specific cell types and their requirements. Basic Cell Culture: A Practical Approach, JM Davis, ed., pp. 227-274, Oxford University Press. 
13. Nagira T Narisawa J Teruya K Katakura Y Shim S Kusumoto K Tokumaru S Tokumaru K Barnes D and Shirahata S 2003, Suppression of UVC-induced cell damage and enhancement of DNA repair by the fermented milk, Kefir. Cytotech, 40, 125-137. 
14. Toumadje, A., K. Kusumoto, A. Parton, P. Mericko, L. Dowell, G. Ma, L. Chen, D.W. Barnes and J.D. Sato 2003 Pluripotent differentiation of murine ES-D3 embryonic stem cells. In Vitro Cell and Developmental Biology 39, 449-453. 
15. Mattingly C., Parton A., Dowell L., Rafferty J. and Barnes D., 2004, Cell and molecular biology of marine elasmobranchs: Squalus acanthias and Raja erinacea. Zebrafish, 1, 111-120.
16. Barnes, D. and Collodi, P., 2005, Fish cell lines and stem cells in The Physiology of Fishes, 3rd edition, Evans, D., Claiborne, J.B. eds, CRC Press, pp.553-575. 
17. Barnes D.W., Mattingly, C.J., Parton, A., Dowell, L.M., Bayne, C.J. and Forrest, J.N. 2005, Marine organism cell biology and regulatory sequence discovery in comparative functional  genomics. Cytotechnology, 43, 123-137. 
18. Barnes, D., Dowell, L., Forest, D., Parton, A., Pavicevic P., and Kazianis, S., 2006, Characterization of XM, a novel Xiphophorus melanoma-derived cell line. Zebrafish 3, 371-381. 
19. Forest, D., Nishikawa, R., Kobayashi, H., Parton, A., Bayne, C.J. and Barnes, D.W., 2007, RNA expression in a cartilaginous fish cell line reveals ancient 3’ noncoding regions highly conserved in vertebrates. Proc. Natl. Acad. Sci. USA, 104, 1224-1229.  
20. Parton, A., Forest, D., Kobayashi, H., Dowell, L.M., Bayne, C.J. and Barnes, D., 2007, Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Comparative Biochemistry and Physiology, 145, 111-119. 
21. Kobayashi, H., Parton, A., Czechanski, A., Durkin, C., Kong, J. and Barnes, D.W., 2007 Multidrug.-resistance-associated protein 3 (Mrp3/Abcc3/Moat-D) is expressed in the SAE Squalus acanthias shark embryo-derived cell line. Zebrafish, 4, 261-275. 
22. Tomana, M., Parton A. and Barnes, D.W., 2008, An Improved method for separation of leukocytes from peripheral blood of the little skate (Leucoraja erinacea). Fish and Shellfish Immunology, 25, 188-190. 
23. Barnes, D.W., Parton, A., Tomana, M., Hwang, J., Czechanski, A. and Collodi, P., 2008, Stem cells from cartilaginous and bony fish. Methods in Cell Biology, 86, 343-67.
24. Hwang, J.-H., Parton, A., Czechanski, A., Ballatori, N.  and Barnes D. 2008, Arachidonic acid-Induced expression of the organic solute and steroid transporter-beta (Ost-beta) in a cartilaginous fish cell line. Comparative Biochemistry and Physiology, Toxicology and Pharmacology, 148, 39-47. 
25. Lage, C.R., Petersen, C.W., Forest, D., Barnes, D., Kornfield, I., Wray, C.  2008,
26. Evidence of multiple paternity in spiny dogfish (Squalus acanthias) broods based on
27. microsatellite analysis.  Journal of Fish Biology, 73, 2068-2074. 
28. Kusumoto, K., Parton, A., Barnes, D. 2009, Mitogen limitation and bone
29. morphogenetic protein-4 promote neurogenesis in SFME cells, an EGF-dependent
30. neural stem cell line, In Vitro Cell and Developmental Biology, 45, 55-61. 
31. Sato, G.H., Sato, J.D., Okamoto, T., McKeehan, W.L., Barnes, D.W. 2010, Tissue culture: the unlimited potential.  In Vitro Cell and Developmental Biology, 46, 590-594. 
32. Parton, A., Bayne, C.M., Barnes, D.W. 2010, Analysis and functional annotation of expressed sequence tags from in vitro cell lines of elasmobranchs: spiny dogfish shark (Squalus acanthias) and little skate (Leucoraja erinacea).  Comparative Biochemistry and Physiology, Part D., Genomics and Proteomics, 5, 199-206. 
33. Barnes, D.W. 2011, Continuously proliferating cell lines from cartilaginous fishes. Advances in Zoology Research, 3, in press. 
34. Barnes, D.W. 2011, Cell and molecular biology of the spiny dogfish shark Squalus acathias and little skate Leucoraja erinacea: insights from in vitro cultured cells.  Journal of Fish Biology, in press.