Biography

Prof. Yuin-Han Jonathan Loh

Institute of Molecular and Cell Biology (IMCB)

Children's Hospital Boston, USA


Email: yhloh@imcb.a-star.edu.sg


Qualifications

2008-2011 Post-doctoral Fellowship, Stem cell Biology, Harvard Medical School

2003-2008 Ph.D., Biological Chemistry and Molecular Pharmacology, National University of Singapore

1999-2002 B.Sc., Cellular and Molecular Biology, National University of Singapore


Publications (Selected)

  1. Fang HT, EL Farran CA, Xing QR, Zhang LF, Li H, Lim B, Loh YH. Global H3.3 Dynamic Deposition Defines its Bimodal Role in Cell Fate Transition. Nat Commun. 2018 April 18;9:1537.
  2. Cheng H, Ang YKH, Li P, Fang HT, EL Farran C, Liu TM, Kong SL, Chin LZM, Lim KH, Li H, Loh YH*, Lim B. Direct Reprogramming of Fibroblasts to Multipotent Hematopoietic Progenitors and Molecular Pathways Recruited. Nat Commun. 2016 Nov 21;7:13396.
  3. Khattar E, Kumar P, Liu CY, Akıncılar SC, Raju A, Lakshmanan M, Maury JJ, Qiang Y, Li S, Tan EY, Hui KM, Shi M, Loh YH, Tergaonkar V. Telomerase reverse transcriptase promotes cancer cell proliferation by augmenting tRNA expression J Clin Invest. 2016 Sep 19. pii: 86042. doi: 10.1172/JCI86042. [Epub ahead of print]
  4. Chen HY, Tan HK, Loh YH*. Derivation of Transgene-Free Induced Pluripotent Stem Cells from a Single Drop of Blood. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:4A.9.1-4A.9.10.
  5. Cheow LF, Courtois ET, Tan Y, Viswanathan R, Xing Q, Tan RZ, Tan DS, Robson P, Loh YH, Quake SR, Burkholder WF. Single-cell multimodal profiling reveals cellular epigenetic heterogeneity. Nat Methods. 2016 Aug 15. doi: 10.1038/nmeth.3961. [Epub ahead of print]
  6. Sivalingam J, Lam AT, Chen HY, Yang BX, Chen AK, Reuveny S, Loh YH, Oh SK. Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platform. Tissue Eng Part C Methods. 2016 Aug;22(8):765-80. doi: 10.1089/ten.TEC.2015.0579. Epub 2016 Aug 1.
  7. Zhang J, Ratanasirintrawoot S, Chandrasekaran S, Wu Z, Ficarro SB, Yu C, Ross CA, Cacchiarelli D, Xia Q, Seligson M, Shinoda G, Xie W, Cahan P, Wang L, Ng SC, Tintara S, Trapnell C, Onder T, Loh YH, Mikkelsen T, Sliz P, Teitell MA, Asara JM, Marto JA, Li H, Collins JJ, Daley GQ. LIN28 Regulates Stem Cell Metabolism and Conversion to Primed Pluripotency. Cell Stem Cell. 2016 Jul 7;19(1):66-80. Epub 2016 Jun 16.
  8. Toh CX, Chan JW, Chong ZS, Wang HF, Guo HC, Satapathy S, Ma D, Goh GY, Khattar E, Yang L, Tergaonkar V, Chang YT, Collins JJ, Daley GQ, Wee KB, Farran CA, Li H, Lim YP, Bard FA, Loh YH*. RNAi Reveals Phase-Specific Global Regulators of Human Somatic Cell Reprogramming. Cell Rep. 2016 Jun 21;15(12):2597-607. Epub 2016 Jun 9.
  9. Zhang W, Ni P, Mou C, Zhang Y, Guo H, Zhao T, Loh YH*, Chen L. Cops2 promotes pluripotency maintenance by Stabilizing Nanog Protein and Repressing Transcription. Sci Rep. 2016 May 26;6:26804.
  10. Seah YF, El Farran CA, Warrier T, Xu J, Loh YH*. Induced Pluripotency and Gene Editing in Disease Modelling: Perspectives and Challenges. Int J Mol Sci. 2015 Dec 2;16(12):28614-34.
  11. Yu S, Ma H, Ow JR, Goh Z, Chiang R, Yang H, Loh YH*, Wu Q. Zfp553 Is Essential for Maintenance and Acquisition of Pluripotency. Stem Cells Dev. 2016 Jan 1;25(1):55-67. Epub 2015 Dec 15.
  12. Yang BX, El Farran CA, Guo HC, Yu T, Fang HT, Wang HF, Schlesinger S, Seah YF, Goh GY, Neo SP, Li Y, Lorincz MC, Tergaonkar V, Lim TM, Chen L, Gunaratne J, Collins JJ, Goff SP, Daley GQ, Li H, Bard FA, Loh YH*. Systematic identification of factors for provirus silencing in embryonic stem cells. Cell. 2015 Sep 24;163(1):230-45. Epub 2015 Sep 10.
  13. Maury JJ, El Farran CA, Ng D, Loh YH, Bi X, Bardor M, Choo AB. RING1B O-GlcNAcylation regulates gene targeting of polycomb repressive complex 1 in human embryonic stem cells. Stem Cell Res. 2015 Jul;15(1):182-9. Epub 2015 Jun 17.
  14. Ma H, Ow JR, Tan BC, Goh Z, Feng B, Loh YH, Fedele M, Li H, Wu Q. The dosage of Patz1 modulates reprogramming process. Sci Rep. 2014 Dec 17;4:7519.
    Faucon PC, Pardee K, Kumar RM, Li H, Loh YH, Wang X. Gene networks of fully connected triads with complete auto-activation enable multistability and stepwise stochastic transitions. PLoS One. 2014 Jul 24;9(7):e102873.
  15. Lu Y, Loh YH*, Li H, Cesana M, Ficarro SB, Parikh JR, Salomonis N, Toh CX, Andreadis ST, Luckey CJ, Collins JJ, Daley GQ, Marto JA. Alternative splicing of MBD2 supports self-renewal in human pluripotent stem cells. Cell Stem Cell. 2014 Jul 3;15(1):92-101. Epub 2014 May 8.
  16. Tan HK, Toh CX, Ma D, Yang B, Liu TM, Lu J, Wong CW, Tan TK, Li H, Syn C, Tan EL, Lim B, Lim YP, Cook SA, Loh YH*. Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking. Stem Cells Transl Med. 2014 May;3(5):586-98. Epub 2014 Mar 19.
  17. Ma H, Ng HM, Teh X, Li H, Lee YH, Chong YM, Loh YH, Collins JJ, Feng B, Yang H, Wu Q. Zfp322a Regulates mouse ES cell pluripotency and enhances reprogramming efficiency. PLoS Genet. 2014 Feb 13;10(2):e1004038.
  18. Wen B, Wu H, Loh YH*, Briem E, Daley GQ, Feinberg AP. Euchromatin islands in large heterochromatin domains are enriched for CTCF binding and differentially DNA-methylated regions. BMC Genomics. 2012 Oct 26;13:566.
  19. Bajpai VK, Mistriotis P, Loh YH, Daley GQ, Andreadis ST. Functional vascular smooth muscle cells derived from human induced pluripotent stem cells via mesenchymal stem cell intermediates. Cardiovasc Res. 2012 Dec 1;96(3):391-400. Epub 2012 Aug 31.
  20. Loh, Y. H., Wu, Q., Chew, J. L., Vega, V. B., Zhang, W., Chen, X., Bourque, G., George, J., Leong, B., Liu, J., Wong, K. Y., Sung, K. W., Lee, C. W., Zhao, X. D., Chiu, K. P., Lipovich, L., Kuznetsov, V. A., Robson, P., Stanton, L. W., Wei, C. L., Ruan, Y., Lim, B., and Ng, H. H. (2006). The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nature Genetics 38, 431–440. Epub 2006 Mar 5.
  21. Agarwal, S., Loh, Y. H., McLoughlin, E. M., Huang, J., Park, I. H., Miller, J. D., Huo, H., Okuka, M., dos Reis, R. M., Loewer, S., Ng, H. H. Keefe, D. L., Goldman, F. D., Klingelhutz, A. J., Liu, L., Daley, G. Q. (2010) Telomere elongation in dyskeratosis congenital induced pluripotent stem cells. Nature464, 292-296. Epub 2010 Feb 17.
  22. Loh, Y. H., Hartung, O., Li, Hu., Guo C., Sahalie, J. M., Manos P. D., Urbach, A., Heffner G. C., Grskovic, M., Vigneault, F., Lensch M. W, Park I. H., Agarwal, S., Church, G. M., Collins, J. J., Irion, S., Daley, G. Q. (2010). Reprogramming of T Cells from Human Peripheral Blood. Cell Stem Cell 7, 15-19.
  23. Loh, Y. H., Zhang, W., Chen, X., George, J., Ng, H. H. (2007). Jmjd1a and Jmjd2c histone H3 lysine 9 demethylases regulate self-renewal in embryonic stem cells. Genes and Development 21, 2545-2557.
  24. Loh, Y. H., Agarwal, S., Park, I. H., Urbach, A., Park, I. H., Heffner, G.C., Kim, K., Miller, J. D., Ng, K., Daley G.Q. (2009). Generation of induced pluripotent stem cells from human blood. Blood. 113, 5476-5479. Epub 2009 Mar 18.
  25. Jiang, J., Chan, Y. S., Loh, Y. H.*, Cai, J., Tong, G. Q., Lim, C. A., Robson, P., Zhong, S., Ng, H. H. (2008). A core Klf circuitry that regulates self-renewal of embryonic stem cells.Nature Cell Biology 10, 353-360. Epub 2008 Feb 10.
  26. Warren, L., Manos, P. D., Ahfeldt, T., Loh, Y. H.*, Lau, F., Ebina, W., Smith, Z. D., Li, H., Collins, J. J., Meissner, A., Daley, GQ., Brack, A. S., Cowan, C., Schlaeger, T. M., Rossi, D.J. (2010). Highly efficient reprogramming to pluripotency and directed differentiation using synthetic mRNA. Cell Stem Cell 7(5):618-30. Epub 2010 Sep 30.
  27. Loh, Y. H.*, Ng, J. H, Ng, H. H. (2008). Molecular framework underlying pluripotency Cell Cycle 7: 7, 1-8. Invited Review.
  28. Loewer, S., Cabili, M., Guttman, M., Loh, Y. H., Thomas, K., Park, I. H., Garber, M., Curran, M., Manos, P. D., Agarwal, S., Onder, T., Datta, S., Lander, E. S., Schlaeger, T. M., Daley, GQ., Rinn, J. L.(2010). Large intergenic non-coding RNAs as novel modulators of reprogramming. Nature Genetics42(12):1113-7. Epub 2010 Nov 7. Paper was featured in Preview by Huck-Hui Ng (Dec 2010)Nature Genetics 42(12).
  29. Chan E. M., Ratanasirintrawoot S., Park I. H., Manos P. D., Loh Y. H., Huo H., Miller J. D., Hartung O., Rho J., Ince T. A., Daley G. Q., Schlaeger T.M. (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nature Biotechnology. 27, 1033-1037. Epub 2009 Oct 11.
  30. Chen, X. Xu, H., Yuan, P., Fang, F., Huss, M., Vega, V., Wong, E., Orlov, Y., Zhang, W., Jiang, J., Loh, Y. H., Yeo, H. C., Yeo, Z. H., Narang, V., Govindarajan, K.R., Leong, B., Shahab, A., Ruan, Y., Bourque, G., Sung, W.K., Clarke, N., Wei, C.L., Ng, H.H. (2008) Integration of external signaling pathways with the core transcriptional network in embryonic stem cells. Cell 13; 133(6):1106-17.
  31. Chew, J. L., Loh, Y. H.*, Zhang, W., Chen, X., Tam, W. L., Yeap, L. S., Li, P., Ang, Y. S., Lim, B., Robson, P., and Ng, H. H (2005). Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Molecular and Cellular Biology 25, 6031-6046.
  32. Lim, L. S., Loh, Y. H.*, Zhang, W., Li, Y., Chen, X., Wang, Y., Bakre, M., Ng, H. H., and Stanton, L. W. (2007). Zic3 Is Required for Maintenance of Pluripotency in Embryonic Stem Cells. Molecular Biology of the Cell 18, 1348-1358. Epub 2007 Jan 31.
  33. Feng, B., Jiang, J., Kraus, P., Ng, J.H., Heng, J.C., Chan, Y.S., Yaw, L.P., Zhang, W., Loh, Y.H., Han, J., Vega, V.B., Cacheux-Rataboul, V., Lim, B., Lufkin, T., Ng, H.H. (2009). Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb. Nature Cell Biology. 11(2):197-203. Epub 2009 Jan 11.
  34. Yuan P., Han J., Guo G., Orlov Y. L., Huss M., Loh Y. H., Yaw L. P., Robson P., Lim B., Ng H.H. Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells. Genes and Development 23, 2507-2520.
  35. Ng, J. H., Heng, J. C. D., Loh, Y. H.*, Ng, H. H. (2008) Epigenetic and Transcriptional regulation of stem cells. Mutat Res. 1; 647(1-2):52-8. Epub 2008 Aug 20. Invited Review.
  36. Rodda, D.J., Chew, J. L., Lim, L. H., Loh, Y. H., Wang, B., Ng, H. H., and Robson, P. (2005). Transcriptional regulation of Nanogby OCT4 and SOX2. Journal of Biological Chemistry 280, 24731–24737. Epub 2005 Apr 27.
  37. Wu, Q., Chen, X., Zhang, J., Loh, Y. H., Low, T. Y., Zhang, W., Zhang, W., Sze, S. K., Lim, B., and Ng, H. H. (2006). Sall4 interacts with Nanog and co-occupies Nanog genomic sites Gore, A., Li, Z., Fung, H. L., Young, J., Agarwal, S., Antosiewicz-Bourget, J., Canto, I., Israel, M., Kiskinis, E., Lee, J. H., Loh, Y. H., Manos P.D., Montserrat, N., Wilbert, M., Yu, J., Kirkness, E., Belmonte, J. C. I, Daley, G. Q., Eggan, K., Rossi, D. J., Thomson, J., Goldstein, L. S. B., Zhang, K. (2010). Somatic coding mutations in human induced pluripotent stem cells. Nature.
  38. Kim, K., Zhao, R., Doi, A., Ng, K., Unternaehrer, J., Cahan, P., Hongguang, H., Loh, Y. H., Aryee, J. M., Feinberg, P. A., Daley, G. Q., Epigenetic memory in human induced pluripotent stem cells. Nature Biotechnology.


Personal Profile

https://www.a-star.edu.sg/imcb/imcb-research/scientific-programmes/cell-biology-and-therapies/jonathan-yuin-han-loh
https://research.a-star.edu.sg/researcher/jonathan-loh-yuin-han/

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