Biodegradation of Kirkuk light crude oil by Bacillus thuringiensis, Northern of Iraq


This study was conducted to identify the viability of Bacillus thuringiensis bacterial on a bio-degradation process for Kirkuk light crude oil. The viable count of Bacillus thuringiensis showed great capability on the biodegradation of crude oil. These bacteria exhibit the ability to dismantle crude oil through clear emulsion layer of crude oil. And they have a good efficiency to dismantle hydrocarbon compounds by 80%, and total biomass reaches to 5 g/l, while the amount of emulsion reaches to 2.3 g/l. For more evidences on the biodegradation action of Bacillus thuringiensis which have been supported by using the technology of gas-Chromatography which confirms the occurring of biodegradation process. The visual examination of gas-Chromatography shows the disappearance of a number of chemicals, as well as decrease in peak area for some material.

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Thamer, M. , Al-Kubaisi, A. , Zahraw, Z. , Abdullah, H. , Hindy, I. and Khadium, A. (2013) Biodegradation of Kirkuk light crude oil by Bacillus thuringiensis, Northern of Iraq. Natural Science, 5, 865-873. doi: 10.4236/ns.2013.57104.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] FAQ (2011) Microbes & oil spills. American Academy of Microbiology, WashingtonDC, 20036.
[2] Das, K. and Mukherjeen, A.K. (2007). Crude petroleum oil biodegradation efficiency of Bacillus subtilis and Ps eudomonas aeruginosa strains isolated from a petroleum oil contaminated soil from North-East India. Journal of Bioresource Technology, 98, 1339-1345.
[3] Latha, R. and Kalaivani, R. (2012) Bacterial degradation of crude oil by gravimetric analysis. Advances in Applied Science Research, 3, 2789-2795.
[4] Elliot, D. (1997) Energy, society and environment. Rout ledge introductions to environment series. 2nd Edition, Routledge, New York.
[5] Farmer, A. (1997) Managing environmental pollution. Routledge Environmental Management series. Routledge, New York.
[6] Atlas, R.M. (1992) Petroleum microbiology. In: Encyclopedia of Microbiology, Academic Press, Baltimore, 363-369.
[7] Lal, B. and Khanna, S. (1996) Degradation of crude oil by Acinetobacter calcoaceticus and Alcaligenes odorans. Journal of Applied Bacteriology, 81, 355-362.
[8] Fred, A.A. (2001) Analyst bacteria for crude oil and some of its derivatives from the soil of southern Iraq. M.S. Thesis, University of Basra, Basra, 135 pages.
[9] Robertson, B., Arhelger, S., Kinney, P.J. and Button, D.K. (1973) Hydrocarbon degradation in Alaskan waters. In: Ahearn, DG. and Meyers, S.P. (Eds.), Microbial Degradation of Oil Pollutants, Louisana State University, 171-184.
[10] AMSA (2004) Management and disposal of oil spill debris. Land farming of oil and oily Debris. Marine environmental protection. AMSAs Role in maritime environ mental issues. Astralian Maritime Safety Authority, 1-24.
[11] Das, N. and Chandran, P. (2011) Microbial degradation of petroleum hydrocarbon contaminants: An overview. Journal of Biotechnology Research International, 2011, 941-810.
[12] Dagly, S. (1984) Introduction in microbial degradation of organic compounds. Marcel Dekker, Inc., New York.
[13] Herman, D.C., Zhang, Y. and Miller, R.M. (1997) Rham nolipid (biosurfactant) effect on cell agreement and bio degradation of residual hexadecane under saturated flow conditions. Journal of Applied and Environmental Micro biology, 63, 3622-3627.
[14] Duvnjak, Z., Cooper, D.G. and Kosaric, N. (1982) Pro duction of surfactant by arthrobacter paraffineus ATCC 19558. Journal of Biotechnology and Bioengineering, 24, 165-175.
[15] Ball, A.S. and Mccarthy, A.J. (1989) Production and pro perties of xalanases from actinomycetes. Journal of Ap plied Bacteriology, 66, 439-444.
[16] Kosaric, N. (2001) Biosurfactants and their application for soil bioremedation. Food Technology and Biotechno logy, 39, 259-304.
[17] Marins, P.D., Carvalho, F.D. and Lippel, S.A. (2002) Bio remediation of clay soils impacted by petroleum. Journal of Engenharia Térmica, 29-32.
[18] Kates, M. (1972) Techniques of lipidology. In: Work, T.S. and Work, E. (Eds.), Techniques of Lipidology: Isolation, Analysis and Identification of Lipids, American Elsvier Publishing, Co., Inc. New York, 269 pages.
[19] Reddy, P.G., Singh, H.D., Pathak, M.G., Bhagat, S.D. and Baruah, J.N. (1983) Isolation and functional characterization of hydrocarbons emulsifying and solubilizing factors produced by a pseudomonas species. Journal of Biotech nology and Bioengineering, 24, 387-401.
[20] Teschner, M. and Wehner, H. (1985) Chromatographic investigation as on biodegraded crude oils. Chromatogra phia, 20, 407-416.
[21] Al-Jubouri, M.K.S. (2004) Role of some cyanobacterial species in biodegradation of some petroleum compounds. M.S. Thesis, Tikrit University, Tikrit, 97 pages.
[22] Al-Khazali, I.H. (2000) Study the efficiency of Pseudomonas aeruginosa bacteria in degradation of hydrocarbon waste and produce of bioemulsions. M.S. Thesis, Bagh dad University, Baghdad, 122 pages.
[23] Nwaogu, L.A., Onyeze, G.O. and Nwabueze, R.N. (2008) Degradation of diesel oil in a polluted soil using Bacillus subtilis. African Journal of Biotechnology, 7, 1939-1943.
[24] Al-Obeidi, A.M.A. (2003) Study bio-degradation and infrared spectrum of Kirkuk crude samples treated by nitrifying cyanobacteria. Tikrit Journal of Pure Science, 9, 52-66.
[25] Hazen, T.C., Tien, A.J., worsztynowicz, A., Altman, D.J., Ulfig, K. and Manko, T. (2003) Biopiles for remediation of petroleum–contaminated soils: Apolish case study. The Utilization of Bioremediation to Reduce Soil Contamination: Problems and Solutions, IV Earth and Environmental Science, NATO Science Series, 19, 229-246.
[26] Pineda-Flores, G. and Mesta-Howard, A.M. (2001) Petroleum asphaltenes: Generated problematic and possible biodegradation mechanisms. Journal of Review Latinoam Microbiologia, 43, 143-150.
[27] Al-Dulaimi, K.S. (2002) Microbial enzymes and bio technology. Part II, Chapter 10, enzymes in the technologies and other industries. Philadelphia University, Jordan, 289-308.
[28] Hanan, I.M., Linda, M.F. and AL-Deeb, T.M. (2009) Mutational analysis of oil degrading genes in bacterial isolates from oil contaminated soil at the Jordanian oil refinery. Journal of World Applied Sciences, 6, 208-220.
[29] Owaid, Y.H. (2008) The common and solitary action of some bacterial isolates on biodegradation of average Kirkuk crude oil. Journal of the Science Rafidain, 19, 101-115.
[30] Anuradha, S.N., Krushi, S.H. and Harish, G.S. (2009) Bioemulsifiers from marine microorganisms. Journal of Scientific & Industrial Research, 68, 273-277.
[31] Cooper, D.G. and Goldenberg, B.G. (1987) Surface active agents from Bacillus species. Journal of Applied and Environmental Microbiology, 53, 224-229.
[32] Suwansukho, P., Rukachisirikul, V., Kawai, F. and Kitti kun, A. (2008) Production and applications of biosurfactant from Bacillus subtilis MUV4. Songklanakarin Journal of Science Technology, 30, 87-93.
[33] Kadarwati, S. and Herlina, L. (2008) Effect of biosurfactant produced by Bacillus. Journal of Lemigas Scientific Contributions, 31, 40-46.
[34] Cybulski, Z., Dziurla, E., Kaczorek, E. and Olszanowski, A. (2003) The influence of emulsifiers on hydrocarbon biodegradation by Pseudomondacea and Bacillacea strains. Spill Science & Technology Bulletin, 8, 503-507.
[35] Wong, J.W.C., Fang, M., Zhao, Z. and Xing, B. (2004) Effect of surfactants on solubilization and degradation of phenenthrene under thermophilic conditions. Journal of Environmental Quality, 33, 2015-2025.
[36] Ahimou, F., Jacques, P. and Deleu, M. (2000) Surfactin and iturin A effects on Bacillus subtilis surface hydro phobicity. Enzyme and Microbial Technology, 27, 749-754. doi:10.1016/S0141-0229(00)00295-7
[37] Maier, R.M. (2003) Biosurfactant: Evolution and diversity in bacteria. Advance in Applied Microbiology, 52, 101-121.
[38] Mukherjee, A.K. and Das, K. (2005) Correlation between diverse cyclic lipopeptides production and regulation of growth and substrate utilization by Bacillus subtilis strains in a particular habitat. FEMS Microbiology Eco logy, 54, 479-489.
[39] Ron, E.Z. and Rosenberg, E. (2001) Natural roles of biosurfactants. Environmental Microbiology, 3, 229-236.
[40] Okerentugba, P.O. and Ezeronye, O.U. (2003) Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria. African Journal of Biotechnology, 2, 288-292.
[41] Sathishkumar, M., Binupriya, A.R., Baik, S. and Yun, S. (2008) Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas. CLEAN-Soil, Air, Water, 36, 92-96.
[42] Qasim, G.M. and Ali, M.A. (1989) Biology of soil microorganisms. Chapter 2, sections of soil microorganism. Ministry of Higher Education and Scientific Research, College of Science.
[43] Okoh, A.I. (2003) Biodegradation of bonny light crude oil in soil microcosm by some bacterial strains isolated from crude oil flow stations saver pits in Nigeria. African Journal of Biotechnology, 2, 104-108.
[44] Muller, R. (2000) Environmental microbiology degradation of environmental pollutants. Part 1: Ecology of microorganisms: Lecture for students of chemical and civil engineering and for students of the master program environmental engineering. Hamburg University of Technology, Hamburg.
[45] Diaz, M.P., Boyd, K.G., Grigson, S.J.W. and Burgess, J.G. (2002) Biodegradation of crude oil across a wide range of salinities by an extremely halotolerant bacterial consortium MPD-M, immobilized onto polypropylene fibers. Biotechnology and Bioengineering, 79, 145-153.
[46] Gilbert, F., Stora, G., Desrosiers, G., Deflandre, B., Bertrand, J.C., Poggiale J.C. and Gange J. P. (2001) Alternation and release of ailphatic compounds by the polychaete Nereisvirens (Sars) experimentally fed with hydrocarbons. Journal of Experimental Marine Biology and Eco logy, 256, 199-213.
[47] Sharma, A. and Rehman, M.B. (2009) Labratory sacle bioremediation of diesel hydrocarbon in soil by indigenous bacterial consortium. Indian Journal of Experimental Biology, 47, 766-769.

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