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Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.

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DOI: 10.4236/abb.2012.33035    3,400 Downloads   6,329 Views   Citations

ABSTRACT

Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Yam-Puc, A. , Avilés-Berzunza, E. , Chan-Bacab, M. , Peña-Rodríguez, L. and Godoy-Hernández, G. (2012) Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.. Advances in Bioscience and Biotechnology, 3, 256-258. doi: 10.4236/abb.2012.33035.

References

[1] Chan-Bacab, M.J., Balanza, E., Deharo, E., Mu?oz, V., García-Durán, R. and Pe?a-Rodriguez, L.M. (2003) Variation of leishmanicidal activity in four populations of Urechites andrieuxii. Ethnopharmacology, 86, 243-247. doi:10.1016/S0378-8741(03)00011-4
[2] Lezama-Dávila, C.M., Isaac-Márquez, A.P., Zamora-Cresencio, P., Uc-Encalada, M.R., Justiniano-Apolinar, S.Y., Angel-Robles, R. del., Satoskar, A. and Hernández-Rivero, L. (2007) Leishmanicidal activity of Urechites andrieuxii. Fitoterapia, 78, 255-257. doi:10.1016/j.fitote.2006.12.005
[3] Tillequin, F., Michael, S. and Seguin, I. (1993) Alkaloids and sulphur compounds. Academic Press, San Diego.
[4] Yam-Puc, A., Chee-González, L., Escalante-Erosa, F., Arunachalampilai, A., Wendt, O.F., Sterner, O., Godoy-Hernández, G. and Pe?a-Rodríguez, L.M. (2012) Steroids from the root extract of Pentalinon andrieuxii. Phyto chemistry Letters, 5, 45-48. doi:10.1016/j.phytol.2011.09.004
[5] Domínguez-Carmona, D.B., Escalante-Erosa, F., García-Sosa, K., Ruíz-Pinelli, G., Gutiérrez-Yapu, D., Chan-Bacab, M.J., Giménez-Turba, A. and Pe?a-Rodríguez, L.M. (2010) Antiprotozoal activity of Betulinic acid derivatives. Phytomedicine, 17, 379-382. doi:10.1016/j.phymed.2009.08.002
[6] Yogeeswari, P. and Sriram, D. (2005) Betulinic acid and its derivatives: A review on their biological properties. Current Medicinal Chemistry, 12, 657-666. doi:10.2174/0929867053202214
[7] Yam-Puc, A., Escalante-Erosa, F., Pech-López, M., Chan-Bacab, M.J., Arunachalampilai, A., Wendt, O.F., Sterner, O. and Pe?a-Rodríguez, L.M. (2009) Trinorsesquiterpenoids from the root extract from Pentalinon andrieuxii Muell-Arg. Journal of Natural Products, 72, 745-748. doi:10.1021/np800554n
[8] Sumiya, T., Ishigami, K. and Watanabe, H. (2010) Stereo- selective total synthsis of (±)—Urechitol A. Angewandte Chemie International Edition, 49, 5527-5528. doi:10.1002/anie.201002505
[9] Phillips, G.C. and Collins, G.B. (1979) In vitro tissue culture of selected legumes and plant regeneration from callus culture of red clover. Crop Science, 19, 59-64. doi:10.2135/cropsci1979.0011183X001900010014x
[10] Zhang, H.X. and Zeevaart, J.A.D. (1999) An efficient Agrobacterium tumefaciens-mediated transformation and regeneration system for cotyledons of spinach (Spinacia oleracea L.). Plant Cell Reports, 18, 640-645. doi:10.1007/s002990050635
[11] Jefferson, R. (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Molecular Biology Reporter, 5, 387-405. doi:10.1007/BF02667740
[12] Humara, J.M., López, M. and Ordas, R.J. (2003) Agrobacterium tumefaciens-mediated transformation of Pinus pinea L. cotyledons: An assessment of factors influencing the efficiency of uidA gen transfer. Plant Cell Report, 19, 51-58. doi:10.1007/s002990050709
[13] Hu, C.Y., Chee, P.P., Chesney, R.H., Zhou, J.H., Miller, P.D. and O′Brien, W.T. (1990) Intrinsic GUS-like activties in seed plants. Plant Cell Reports, 9, 1-5. doi:10.1007/BF00232123
[14] Rezmer, C., Schlichting, R., Wachter, R. and Ullrich, C.I. (1999) Identification and localization of transformed cells in Agrobacterium tumefaciens-induced plant tumors. Planta, 209, 399-405. doi:10.1007/s004250050742
[15] Godoy-Hernández, G., Avilés-Berzunza, E., Carrillo-Pech M. and Vázquez-Flota, F. (2008) Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.). Electronic Journal of Biotech- nology, 11, 1-5. http://158.251.16.248/content/vol11/issue1/full/3/3.pdf
[16] Solís-Ramos, L.Y., González-Estrada, T., Andrade-Torres, A., Godoy-Hernández, G. and Casta?o de la Serna, E. (2010) Endogenous GUS-like activity in Capsicum chinense Jacq. Electronic Journal of Biotechnology, 13, 1-7. http://redalyc.uaemex.mx/redalyc/pdf/1733/173316385002.pdf
[17] Martin-Acosta, J.C., Avilés-Berzunza, E. and Godoy-Hernández, G. (2012) In vitro plant regeneration from explants of Pentalinon andrieuxii (Müll. Arg.). Hansen & Wunderlin, Unpublished.

  
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