Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology


The optimum total carotenoids (TC) extraction from rapeseed with solvent extraction method by UV-visible spectrophotometer determination was investigated by using response surface methodology (RSM). Extraction duration, re-peated extraction cycles, solvent-solid ratio and extraction temperature were assumed to be the most important factors affecting solvent extrac-tion for the determination of TC. Optimum sol-vent extraction conditions for maximizing the determination of TC were: extraction duration 7.3h, repeated extraction three times, ratio of solvent-solid (v/w, mL/mg) 29:1, extraction temperature 42°C. Under the optimal conditions, the yield of TC was up to 4.79 mg /100g. The model had a satisfactory coefficient of R2 (= 0.912) and verified experimentally. The results showed that the conditions were mild and use-ful for maximizing a quantitative spectropho-tometer determination of TC in rapeseed.

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Wang, L. and Liu, Y. (2009) Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology. Natural Science, 1, 23-29. doi: 10.4236/ns.2009.11005.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Holden, J. M., Eldridge, A. L. and Beeeher, G. R. (1999) Carotenoid content of U.S. food: An update of the data-base. Journal of Food Composition and Analysis, 12(3), 169-196.
[2] Sotirios, K. and Vassiliki, O. (2006) Antioxidant proper-ties of natural carotenoid extracts against the AAPH-ini-tiated oxidation of food emulsions. Innovative Food Sci-ence and Emerging Technologies, 7(1), 132-139.
[3] Jamal, J. and Chieri, K. (2006) Variation of lycopene, antioxidant activity, total soluble solids and weight loss of tomato during post harvest storage. Post harvest Biol-ogy and Technology, 41(2), 151-155.
[4] Rao, A. V. and Rao, L. G. (2007) Carotenoids and human health. Pharmacological Research, 55(3), 207-216.
[5] Meléndez-Martínez, A. J., George, B. and Isabel, M. V. (2007) Relationship between the colour and the chemical structure of carotenoid pigments. Food Chemistry, 101(3), 1145-1150.
[6] Meléndez-Martínez, A. J., Vicario, I. M. and Heredia, F. J. (2007) Review: Analysis of carotenoids in orange juice. Journal of Food Composition and Analysis, 20(7), 638-649.
[7] Andrew, J. and Young, G. M. (2001) Antioxidant and prooxidant properties of carotenoids. Archives of Bio-chemistry and Biophysics, 385(1), 20-27.
[8] Adetayo, O. O. and Aluko, R. E. (2005) The anti-car-cinogenic and anti-atherogenic effects of lycopene: A re-view. Trends Food Sci. Technol., 16(8), 344-350.
[9] Cano, A., Acosta, M. and Arnao, M. B. (2003) Hydro-philic and lipophilic antioxidant activity changes during on-vine ripening of tomatoes (Lycopersicon esculentum Mill). Post-harvest Biol. Technol., 28(1), 59-65.
[10] Akhtar, M. H. and Bryan, M. (2008) Extraction and quantification of major carotenoids in processed foods and supplements by liquid chromatography. Food Chem-istry, 111(1), 255-261.
[11] Meléndez-Martínez, A. J., Vicario, I. M. and Heredia, F. J. (2007) Provitamin A, carotenoids and ascorbic acid con-tents of the different types of orange juices marketed in Spain. Food Chemistry, 101(1), 177-184.
[12] Gua, Z. X., Chen, D. M., Han, Y. B., Chen, Z. G. and Gu, F. R. (2008) Optimization of carotenoids extraction from Rhodobacter sphaeroides. LWT-Food Science and Tech-nology, 41(6), 1082-1088.
[13] FAO (2006) Statistical year book. Food and Agriculture Organization of the United Nations, 2/1,2/2, Rome.
[14] Haila, H. and Heinonen, M. (1994) Action of β-carotene on purified rapeseed oil during light storage. Food Science and Technology, 27(6), 573-577.
[15] Hornero-Méndez, D. and Mínguez-Mosquera, M. I. (2007) Bioaccessibility of carotenes from carrots: Effect of cooking and addition of oil. Innovative Food Science & Emerging Technologies, 8(3), 407-412.
[16] Rodriguez-Amaya, D. B., Kimura, M., Godoy, H. T., and Amaya-Farfan, J. (2008) Updated Brazilian database on food carotenoids: Factors affecting carotenoid composi-tion. Journal of Food Composition and Analysis, 21(6), 445-463.
[17] Simkin, A. J., Moreau, H., Kuntz, M., Pagny, G., Chen-wei, L., Tanksley, Se., and McCarthy, J. (2008) An inves-tigation of carotenoid biosynthesis in Coffea canephora and Coffea Arabica. Journal of Plant Physiology, 165(10), 1087-1106.
[18] Sachindra, N. M. and Mahendrakar, N. S. (2005) Process optimization for extraction of carotenoids from shrimp waste with vegetable oils. Bioresource Technology, 96(10), 1195-1200.
[19] Sheetal, M. C. and Laxmi (2007) Enzyme aided extrac-tion of lycopene from tomato tissues. Food Chemistry, 102(1), 77-81.
[20] Huang, W., Li, Z. S., Niu, H., Li, D. and Zhang, J. (2008) Optimization of operating parameters for supercritical carbon dioxide extraction of lycopene by response sur-face methodology. Journal of Food Engineering, 89(3), 298-302.
[21] Mateose, R. and Garcia-Mesa, J. A. (2006) Rapid and quantitative extraction method for the determination of chlorophylls and carotenoids in olive oil by high-performance liquid chromatography. Analytical and Bioanalytical chemistry, 385(7), 1247-1254.
[22] Rodriguez-Amaya, D. B. (2001) A guide to carotenoid analysis in foods. Washington, DC.: Ed. ILSI-Interna-tional Life Sciences Institute.
[23] Schoefs, B. (2002) Chlorophyll and carotenoid analysis in food products. Properties of the pigments and methods of analysis. Trends in Food Science and Technolog., 13(11), 361-371.

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