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Effect of natural and synthetic growth stimulators on in vitro rooting and acclimatization of common ash (Fraxinus excelsior L.) microplants

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DOI: 10.4236/ns.2013.510134    3,414 Downloads   5,192 Views   Citations

ABSTRACT

Application of growth stimulators can be especially effective on plantlets in vitro of tree species which are usually worse rooted and adapted in comparison with annual plants. In our work we evaluate effects of natural (dihydroquercetin, Zircon) and synthetic growth stimulators (Melafen, Fumar, Epin-Extra) on rooting and acclimatization of common ash (Fraxinus excelsior L.) microplants. The 0.05% - 0.2% Zircon and 10-5% Melafen enhanced in vitro rooting by 29% - 37% and 31%, respectively. Melafen also stimulated root formation faster compared to control plants. The dihydroquercetin concentration of 0.01% increased rooting by 24% and root number per shoot by 1.8 times. In vitro plants rooted on media supplemented with Melafen, Fumar and Zircon demonstrated enhanced ability to adapt to non-sterile conditions and accelerated growth. Two months after planting to the greenhouse, plants rooted on 0.01% dihydroquercetin were 45% taller than the control. Weekly spraying of plantlets with 0.02% Epin-Extra containing 24-epibrassinolid stimulated growth of uniform plants with large leaves. The obtained results support the use of growth stimulators for application in clonal micropropagation of common ash both for large-scale production of planting stock and for conservation of rare and valuable genotypes.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Lebedev, V. and Schestibratov, K. (2013) Effect of natural and synthetic growth stimulators on in vitro rooting and acclimatization of common ash (Fraxinus excelsior L.) microplants. Natural Science, 5, 1095-1101. doi: 10.4236/ns.2013.510134.

References

[1] FAO (2004) Preliminary review of biotechnology in forestry, including genetic modification. Rome.
[2] Pijut, P.M., Lawson, S.S. and Michler, C.H. (2011) Biotechnological efforts for preserving and enhancing temperate hardwood tree biodiversity, health, and productivity. In Vitro Cellular and Developmental Biology—Plant, 47, 123-147. http://dx.doi.org/10.1007/s11627-010-9332-5
[3] Durkovic, J. and Misalova, A. (2008) Micropropagation of temperate noble hardwoods: An overview. Functional Plant Science and Biotechnology, 2, 1-19.
[4] Gaspar, T., Kevers, C., Penel, C., Greppin, H., Reid, D.M. and Thorpe, T.A. (1996) Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cellular and Developmental Biology—Plant, 32, 272-289. http://dx.doi.org/10.1007/BF02822700
[5] Chandra, S., Bandopadhyay, R., Kumar, V. and Chandra, R. (2010) Acclimatization of tissue cultured plantlets: From laboratory to land. Biotechnology Letters, 32, 11991205.
http://dx.doi.org/10.1007/s10529-010-0290-0
[6] Kozai, T. (1991) Photoautotrophic micropropagation. In Vitro Cellular and Developmental Biology—Plant, 27, 47-51. http://dx.doi.org/10.1007/BF02632127
[7] Hazarika, B.N. (2003) Acclimatization of tissue-cultured plants. Current Science, 85, 1704-1712.
[8] Azcon-Aguilar, C., Cantos, M., Troncoso, A. and Barea, J.M. (1997) Beneficial effect of arbuscular mycorrhizas on acclimatization of micropropagated plantlets. Scientia Horticulturae, 72, 63-71. http://dx.doi.org/10.1016/S0304-4238(97)00120-9
[9] Reddy, B.O., Giridhar, P. and Ravishankar, G.A. (2002) The effect of triacontanol on micropropagation of Capsicum frutescens and Decalepis hamiltonii W and A. Plant Cell Tissue and Organ Culture, 71, 253-258. http://dx.doi.org/10.1023/A:1020342127386
[10] Nge, K.L., New, N., Chandrkrachange, S. and Stevens, W. F. (2006) Chitosan as a growth stimulator in orchid tissue culture. Plant Science, 170, 1185-1190.
http://dx.doi.org/10.1016/j.plantsci.2006.02.006
[11] Baldotto, L.E.B., Baldotto, M.A., Canellas, L.P., BressanSmith, R. and Olivares, F.L. (2010) Growth promotion of pineapple “Vitoria” by humic acids and Burkholderia spp. during acclimatization. Revista Brasileira de Ciência do Solo, 34, 1593-1600. http://dx.doi.org/10.1590/S0100-06832010000500012
[12] Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
[13] Lloyd, G. and McCown, B.H. (1980) Commercially feasible micropropagation of mountain laurel, (Kalmia latifolia) by use of shoot tip culture. Combined Proceedings of International Plant Propagators’ Society, 30, 421-427.
[14] Gazaryan, I.G. and Lagrimini, L.M. (1996) Tobacco anionic peroxidase overexpressed in transgenic plants: Aerobic oxidation of indole-3-acetic acid. Phytochemistry, 42, 1271-1278.
http://dx.doi.org/10.1016/0031-9422(96)00096-9
[15] Salvador, V.H., Lima, R.B., dos Santos, W.D., Soares, A.R., Bohm, P.A.F., Marchiosi, R., Ferrarese, M.L.L. and Ferrarese-Filho, O. (2013) Cinnamic acid increases lignin production and inhibits soybean root growth. PLoS ONE, 8, e69105. http://dx.doi.org/10.1371/journal.pone.0069105
[16] Osipenkova, O.V., Ermokhina, O.V., Belkina, G.G., Oleskina, Yu.P., Fattakhov, S.G. and Yurina, N.P. (2008) Effect of melaphene on mxpression of Elip1 and Elip2 genes encoding chloroplast light-induced stress proteins in barley. Applied Biochemistry and Microbiology, 44, 635-641. http://dx.doi.org/10.1134/S0003683808060136
[17] Weidmann, A.E. (2012) Dihydroquercetin: More than just an impurity? European Journal of Pharmacology, 684, 19-26. http://dx.doi.org/10.1016/j.ejphar.2012.03.035
[18] Nurminsky, V.N., Ozolina, N.V., Sapega, J.G., Zheleznykh, A.O., Pradedova, E.V., Korzun, A.M. and Salyaev, R.K. (2009) The effect of dihydroquercetin on active and passive ion transport systems in plant vacuolar membrane. Biology Bulletin, 36, 1-5. http://dx.doi.org/10.1134/S1062359009010014
[19] Skadhauge, B., Thomsen, K.K. and von Wettstein, D. (1997) The role of the barley testa layer and its flavonoid content in resistance to Fusarium infections. Hereditas, 126, 147-160.
http://dx.doi.org/10.1111/j.1601-5223.1997.00147.x
[20] Ladyzhenskaya, E.P., Platonova, T.A., Evsyunina, A.S., Fattakhov, S.G., Korableva, N.P. and Reznik, V.S. (2007) The effect of melamine salt of bis(oxymethyl)phosphinic acid (melafen) on the growth processes and plasma membrane function in potato tuber cells. Applied Biochemistry and Microbiology, 43, 222-226. http://dx.doi.org/10.1134/S0003683807020172
[21] Laugale, V. and Daugavietis, M. (2009) Effect of coniferous needle products on strawberry plant development, productivity and spreading of pests and diseases. Acta Horticulturae, 842, 239-242.
[22] Khripach, V.A., Zhabinskii, V.N. and de-Groot, A.E. (2000) Twenty years of brassinosteroids: Steroidal plant hormones warrant better crops for the XXI century. Annals of Botany, 86, 441-447. http://dx.doi.org/10.1006/anbo.2000.1227
[23] Sharma, I., Ching, E., Saini, S., Bhardwaj, R. and Pati, P.K. (2013) Exogenous application of brassinosteroid offers tolerance to salinity by altering stress responses in rice variety Pusa Basmati-1. Plant Physiology and Biochemistry, 69, 17-26. http://dx.doi.org/10.1016/j.plaphy.2013.04.013
[24] Gális, I., Simek, P., Van Onckelen, H.A., Kakiuchi, Y. and Wabiko, H. (2002) Resistance of transgenic tobacco seedlings expressing the Agrobacterium tumefaciens C58-6b gene, to growth-inhibitory levels of cytokinin is associated with elevated IAA levels and activation of phenylpropanoid metabolism. Plant and Cell Physiology, 43, 939-950. http://dx.doi.org/10.1093/pcp/pcf112

  
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