Rootstocks effect on plant nutrition concentration in different organ of grafted watermelon

Halit Yetisir, A. Erhan Özdemir, Veysel Aras, Elif Candır, Ömer Aslan

Alata Horticultural Research Institute Erdemli, Mersin, Turkey.
Department of Horticulture, Faculty of Agriculture, University of Erciyes, Kayseri, Turkey.
Department of Horticulture, Faculty of Agriculture, University of Mustafa Kemal, Hatay, Turkey.
Ministry of Agriculture and Rural Affair, Silifke District Directorate Silifke, Mersin, Turkey.

DOI: 10.4236/as.2013.45033                

Abstract

In this study carried out in 2009 and 2010 growing seasons, effect of rootstocks on plant nutrition partitioning in grafted watermelon onto four gourd rootstocks was investigated. Ferro, RS841 (Cucurbita maxima x C. moschata) and Argentario and Macis (Lagenaria hybrid) were used as rootstocks. Crimson Tide and Crisby watermelon cultivars were used as scion. Grafted and ungrafted seedlings were produced by a commercial seedling company. Plants were grafted by one cotyledon grafting techniques. Experiment was conducted in Alata Horticultural Research Station inMersin. The grafted plants were planted under low tunnel in early spring and regular cultural practices for watermelon were applied. Plant nutrition concentrations were determined in leaf, fruit rind, fruit flesh and seeds. Leaf sample was taken at flowering stage, rind, flesh and seed sample were taken from fully mature fruit. Nitrogen concentration was determined by modified Kjheldahl methods. Phosphorous concentration was determined by vanadomolybdophosphoric acid method. K, Ca, Mg, Fe, Mn, Zn and Cu concentration of samples were determined by atomic absorption spectrophotometry. Plant nutrient concentration in leaf, rind, flesh and seed were significantly affected by rootstocks. Increase in concentration of N, P, K, Ca and Mg in leaves was not observed in grafted plant. Ungrafted plant had higher concentration of Fe, Mn, Zn and Cu in their leaves. Ca concentration in rind of fruits from grafted watermelon was higher than ungrafted control plant except Crimson Tide/Macis and Crimson Tide/Argentario graft combinations. Plant nutrition content of fruit flesh was significantly affected by rootstocks and scion. Magnesium, Fe, Zn and Mn concentration of seed was not significantly influenced by rootstocks and scion while other plant nutrient content was significantly affected rootstocks and scion.

Keywords

Watermelon; Grafting; Rootstocks; Plant Nutrition Partitioning

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	FAO (2011) Statistical Database. www.fao.org.com. 16.08.2011.
[2]	Messiaen, C.M. (1974) Le Potager Tropical (1généralités). Agence de Coop., Culturelle et Technique Publisher, Paris.
[3]	Lee, J.M. (1994). Cultivation of grafted vegetables. I. Current status, grafting methods and benefits, HortScience, 29, 235-239.
[4]	Oda, M. (1995) New grafting methods for fruit–bearing vegetables in Japan. Japan Agricultural Research Quarterly, 29, 187-198.
[5]	Lee, J.M. and Oda, M. (2003) Grafting of herbaceous vegetable and ornamental crops. Horticultural Reviews, 28, 61-124.
[6]	Yetisir, H., Sari, N. and Yücel, S. (2003) Rootstock resistance to fsarium wilt and effect on watermelon fruit yield and quality. Phytoparasitica, 31, 163-169. doi:10.1007/BF02980786
[7]	Yetisir., H. and Sari, N. (2003) Effect of different rootstock on plant growth, yield and quality of watermelon. Australian Journal of Experimental Agriculture, 43, 1269-1274. doi:10.1071/EA02095
[8]	Lopez-Galarza, S.A., San Bautista, D.M., Perez, D.M., Miguel, A., Baixauli, C., Pascual, B, Maroto, J.V. and Guardiola, J.L. (2004) Effect of grafting and cytokinin induced fruit setting on color and sugar content traits in glasshousegrown triploid watermelon. Journal of Horticultural Science and Biotechnology, 79, 971-976.
[9]	Yetisir, H., Kurt, S., Sari N. and Tok, M.F. (2007) Rootstock potential of Turkish Lagenaria siceraria germplasm for watermelon: Plant growth, graft compatibility, and resistance to Fusarium. Turkish Journal of Agriculture and Forestry, 31, 381-388.
[10]	Pulgar, G., Villora, G., Moreno, D.A. and Romero, L. (2000) Improving the mineral nutrition in grafted watermelon plants: Nitrogen metabolism. Plant Biology, 43, 607-609. doi:10.1023/A:1002856117053
[11]	Karaca., F.,Yetisir, H., Solmaz, I., Candir, E., Kurt, S., Sari, N. and Guler, Z. (2012) Rootstock potential of Turkish Lagenaria siceraria germplasm for watermelon: plant growth, yield and quality. Turkish Journal of Agriculture and Forestry, 36, 167-177.
[12]	Bulder, H.A.M., van Hasselt, P.R., Kuiper, P.J.C., Speek, E.J. and den Nijs, A.P.M. (1990) The effect of low root temperature in growth and lipid composition of low temperature tolerant rootstock genotypes for cucumber. Journal of Plant Physiology, 138, 661-666. doi:10.1016/S0176-1617(11)81312-X
[13]	Rivero, R.M., Ruiz, J.M., Sanchez, E. and Romero, L. (2003) Does grafting provide tomato plants and advantages against H2O2 production under conditions of thermal shock? Plant Physiology, 117, 44-50. doi:10.1034/j.1399-3054.2003.1170105.x
[14]	Romero, L., Belakbir, A., Ragala, L. and Ruiz, M. (1997) Response of plant yield and leaf pigments to saline conditions: Effectiveness of different rootstocks in melon plants (Cucumis melo L.). Soil Science and Plant Nutrition, 43, 855-862. doi:10.1080/00380768.1997.10414652
[15]	Yetisir, H. and Uygur, V. (2010) Responses of grafted watermelon onto different gourd species to salinity stress. Journal of Plant Nutrition, 33, 315-327. doi:10.1080/01904160903470372
[16]	Ruiz, J.M., Belakbir, A., Lopez-Cantarero, A. and Romero, L. (1997) Leaf macronutrient content and yield in grafted melon plants: A model to evaluate the influence of rootstocks to genotype. Scientia Horticulturae, 71, 113-123.
[17]	Cohen, S. and Naor, A. (2002) The effect of three rootstocks on water use canopy conductance and hydraulic parameters of apple trees and predicting canopy from hydraulic conductance. Plant, Cell and Environment, 25, 17-28. doi:10.1046/j.1365-3040.2002.00795.x
[18]	Rouphael, Y., Cardarelli, M., Reab, E. and Colla, G. (2008) Grafting of cucumber as a means to minimize copper toxicity. Environ. Journal of Experimental Botany, 63, 49-58. doi:10.1016/j.envexpbot.2007.10.015
[19]	Colla, G., Rouphaelb, Y., Cardarelli, M., Salernoc, A. and Reac, E. (2010) The effectiveness of grafting to improve alkalinity tolerance in watermelon. Environmental and Experimental Botany, 68, 283-291. doi:10.1016/j.envexpbot.2009.12.005
[20]	Yetisir, H., Caliskan M.E, Soylu, S. and Sakar M. (2006) Some physiological and growth responses of watermelon [Cit-rullus lanatus (Thunb.) Matsum and Nakai] grafted onto Lagenaria siceraria to flooding. Environmental and Experimental Botany, 58, 1-3. doi:10.1016/j.envexpbot.2005.06.010
[21]	Yetisir, H. (2001) Karpuzda asili fide kullaniminin bitki büyümesi, verim ve meyve kalitesi üzerine etkileri ile asi yerinin histolojik acidan incelenmesi, Ph.D. Thesis, Cukurova University, Adana.
[22]	Uygur, V. and Yetisir, H. (2009) Effects of rootstocks on some growth parameters, phosphorous and nitrogen uptake watermelon under salt stress. Journal of Plant Nutrition, 32, 629-643. doi:10.1080/01904160802715448
[23]	Curuk, S., Dasgan, H.Y., Mansuroglu S., Kurt, S., Mazmanoglu, M., Tarla, G. and Durgac, C. (2010) Leaf mineral composition of grafted eggplant grown in soil infested with verticillium and root-knot nematodes. Pesquisa Agropecuaria Brasileira, 45, 879-885.
[24]	Colla, G., Suarez, C.M.C., Cardarelli, M. and Rouphael, Y. (2010) Improving nitrogen use efficiency in melon by grafting. HortScience, 45, 559-565.
[25]	SAS (2006) Institute, SAS Online Doc, Version 8. SAS Inst Cary.
[26]	Bergmann, W. (1992) Nutritional disorders of plant-development, visual and analytical diagnosis.
[27]	Heo, Y.C. (1991) Effects of rootstocks on exudation and mineral elements contents in different parts of Oriental melon and cucumber (in Korean with English summary). M.S. thesis, Kyung Hee University, Seoul.
[28]	Jang, K.U. (1992) Utilization of sap and fruit juice of Luffa cylindrica L. Res. Rpt., Korean Ginseng and Tobacco Institute, Taejon.
[29]	Chaplin, M.H. and Westwood, M.N. (1980) Nutritional status of “Bartlett” pear on Cydonia and Pyrus species rootstocks. Journal of American Society Horticultural Science, 105, 60-63.
[30]	Tagliavani, M., Bassi, D. and Marangoni, B. (1993) Growth and mineral nutrition of pear rootstocks in lime soils. Scientia Horticulturae, 54, 13-22. doi:10.1016/0304-4238(93)90079-6
[31]	Brown, P.H., Zhang, Q. and Ferguson, L. (1994) Influence of rootstock on nutrient acquisition by pistachio, Journal of Plant Nutrition, 17, 1137-1148. doi:10.1080/01904169409364794
[32]	Kawaguchi, M., Taji, A., Backhouse, D. and Oda, M. (2008) Anatomy and physiology of graft incompatibility in solanaceous plants. Journal of Horticultural Science and Biotechnology, 83, 581-588.
[33]	Fernández-García, N., Carvajal, M. and Olmos, E. (2004) Graft union formation in tomato plants. Peroxidase and catalase involvement. Annals of Botany, 93, 53-60. doi:10.1093/aob/mch014
[34]	Goreta, S., Bucevic-Popovic, V., Selak, G.V., PavelaVrancic, M. and Perica, S. (2008) Vegetative growth, superoxide dismutase activity and ion concentration of salt-stressed watermelon as influenced by rootstock. Journal of Agricultural Science, 146, 695-704. doi:10.1017/S0021859608007855
[35]	Savvas, D., Pa-pastavrou, D., Ntatsi, G., Ropokis, A., Olympios, C., Hartmann, H. and Schwarz, D. (2009) Interactive effects of grafting and manganese supply on growth, yield, and nutrient uptake by tomato. HortScience, 44, 1978-1982.
[36]	Huang, Y., Bie, Z., He, S., Hua, B., Zhen, A. and Liu, Z. (2010) Improving cucumber tolerance to major nutrients induced salinity by grafting onto Cucurbita ficifolia. Env?ronmental and Experimental Botany, 69, 32-38. doi:10.1016/j.envexpbot.2010.02.002
[37]	Carmen Mar-tínez-Ballesta, M., Alcaraz-Lópeza, C., Muriesa, B., Mota-Cadenasa, C. and Carvajal, M. (2010) Physiological aspects of rootstockscion interactions. Scientia Horti-culturae, 127, 112-118. doi:10.1016/j.scienta.2010.08.002
[38]	Scott, W.D., McCraw, B.D., Motes, J.E. and Smith, M.W. (1993) Ap-plication of calcium to soil and cultivar affect elemental concentration of watermelon leaf and rind tissue. Journal of American Society Horticultural Science, 118, 201-206.
[39]	Salam, M.A., Masum, A.S.M.H., Chow-dhury, S.S., Dhar, M., Saddeque, A. and Islam, M.R. (2002) Growth and yield of watermelon as influenced by grafting. Journal of Biological Sciences, 2, 298-299. doi:10.3923/jbs.2002.298.299
[40]	Davis, A.R. and Per-kins-Veazie, P. (2005) Rootstock effects on plant vigor and watermelon fruit quality. Cucurbit Genet. Cucurbit Genetics Cooperative Reports, 28-29, 39-42.
[41]	Colla, G., Raupahel, Y., Gardarelli, M. and Rea, E. (2006) Effect of salinity on yield fruit quality, leaf gas exchange and mineral composition of grafted watermelon plants. HortScience, 41, 622-627.