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The Use of Dried Blood Spot Samples in Screening Drugs of Abuse

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DOI: 10.4236/pp.2013.42022    5,252 Downloads   8,559 Views   Citations

ABSTRACT

The present article will provide an overview of use of dried blood sampling method for analysis of drug of abuse. Relatively short half life and instability in blood, calls for alternative sampling method for determination of drugs of abuse. Dried blood spot (DBS) method has many advantages over the conventional sampling methods. The available method for DBS sample collection, storage and transport are described here. The techniques involved in and the factors that may influence the accuracy and reproducibility of the DBS methods for determination of drugs of abuse are presented. The DBS sampling has the potential to be a useful technique to detect drugs of abuse. The use of DBS for any drug should be judged against the potential error involved with the method.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

R. Quraishi, R. Jain and A. Ambekar, "The Use of Dried Blood Spot Samples in Screening Drugs of Abuse," Pharmacology & Pharmacy, Vol. 4 No. 2, 2013, pp. 152-159. doi: 10.4236/pp.2013.42022.

References

[1] E. J. Cone, “New Developments in Biological Measures of Drug Prevalence,” NIDA Research Monograph, Vol. 167, 1997, pp. 108-129.
[2] O. H. Drummer, “Postmortem Toxicology of Drugs of Abuse,” Forensic Science International, Vol. 142, No. 2, 2004, pp. 101-113. doi:10.1016/j.forsciint.2004.02.013
[3] P. Holmgren, H. Druid, A. Holmgren and J. Ahlner, “Stability of Drugs in Stored Post Mortem Femoral Blood and Vitreous Humor,” Journal of Forensic Sciences, Vol. 49, No. 4, 2004, pp. 820-825. doi:10.1520/JFS2003433
[4] World Health Organization United Nations Office on Drugs and Crime (UNODC), “Substitution Maintenance Therapy in the Management of Opioid Dependence and HIV/AIDS Prevention,” United Nations Organisation, Geneva, 2004.
[5] J. V. Mei, J. R. Alexander, B. W. Adam and W. H. Hannon, “Use of Filter Paper for the Collection and Analysis of Human Whole Blood Specimens,” Journal of Nutrition, Vol. 131, No. 5, 2001, pp. 1631S-1636S.
[6] D. Brambilla, C. Jennings, G. Aldrovandi, J. Bremer, A. M. Comeau, S. A. Cassol, R. Dickover, J. B. Jackson, J. Pitt, J. L. Sullivan, A. Butcher, L. Grosso, P. Reichelderfer and S. A. Fiscus, “Multicenter Evaluation of Use of Dried Blood and Plasma Spot Specimens in Quantitative Assays for Human Immunodeficiency Virus RNA: Measurement, Precision and RNA Stability,” Journal of Clinical Microbiology, Vol. 41, No. 5, 2003, pp. 1888-1893. doi:10.1128/JCM.41.5.1888-1893.2003
[7] R. Guthrie and A. Susi, “A Simple Method for Detecting Phenylketonuria in Large Populations of Newborn Infants,” Pediatrics, Vol. 32, No. 3, 1963, pp. 338-343.
[8] S. P. Parker and W. D. Cubit, “The Use of Dried Blood Samples in Epidemiological Studies,” Journal of Clinical Pathology, Vol. 52, 1999, pp. 633-639. doi:10.1136/jcp.52.9.633
[9] B. Wilcken and V. Wiley, “Newborn Screening,” Pathology, Vol. 40, No. 2, 2008, pp. 104-115. doi:10.1080/00313020701813743
[10] P. M. Edelbroek, J. van der Heijden and L. M. Stolk, “Dried Blood Spot Methods in Therapeutic Drug Monitoring: Methods, Assays and Pitfalls,” Therapeutic Drug Monitoring, Vol. 31, No. 3, 2009, pp. 327-336. doi:10.1097/FTD.0b013e31819e91ce
[11] T. W. McDade, S. Williams and J. J. Snodgrass, “What a Drop Can Do: Dried Blood Spots as a Minimally Invasive Method for Integrating Biomarkers into Population Based Research,” Demography, Vol. 44, No. 4, 2007, pp. 899- 925. doi:10.1353/dem.2007.0038
[12] M. Barfield, N. Spooner, R. Lad, S. Parry and S. Fowles, “Application of Dried Blood Spots Combined with HPLC-MS/MS for the Quantitation of Acetaminophen in Toxicokinetic Studies,” Journal of Chromatography B Analytical Technologies Biomedical and Life Sciences, Vol. 870, No. 1, 2008, pp. 32-37. doi:10.1016/j.jchromb.2008.05.025
[13] N. Spooner, R. Lad and M. Barfield, “Dried Blood Spots as a Sample Collection Technique for the Determination of Pharmacokinetics in Clinical Studies: Consideration for the Validation of a Quantitative Bioanalytical Method,” Analytical Chemistry, Vol. 81, No. 4, 2009, pp. 1557-1563. doi:10.1021/ac8022839
[14] W. Li and F. L. S. Tse, “Dried Blood Spot Sampling in Combination with LC-MS/MS for Quantitative Analysis of Small Molecules,” Biomedical Chromatography, Vol. 24, No. 1, 2010, pp. 49-65. doi:10.1002/bmc.1367
[15] S. D. O’Broin, B. P. Kelleher and E. Gunter, “Evaluation of Factors Influcing Precision in the Analysis of Samples Taken from Blood Spots on Filter Paper,” Clinical and Laboratory Hematology, Vol. 17, No. 2, 1995, pp. 185- 188.
[16] S. D. O’Broin, “Influence of Hematocrit on Quantitative Analysis of Blood Spots on Filter Paper,” Clinical Chemistry, Vol. 39, No. 6, 1993, pp. 1354-1355.
[17] W. H. Hannon, J. Boyle, B. Davin, A. Marsden, E. R. B. McCabe, M. Schwartz, G. Scholl, B. L. Therrell, M. Wolfson and F. Yoder, “Blood Collection on Filter Paper for Neonatal Screening Programs, Approved Standard,” Document A4A3, 3rd Edition, National Committee for Clinical laboratory Standards, Wayne, 1997.
[18] R. Knudsen, W. Slazyk and J. Riehmond, “Guidelines for the Shipment of Dried Blood Spots Specimens,” Centers for Disease Control and Prevention, Atlanta, 1995. http://www.cdc.gov/od/ohs/biosftydriblood.htm.
[19] A. A. Alfazil and R. A. Anderson, “Stability of Benzodiazepines and Cocaine in Blood Spots Stored on Filter Paper,” Journal of Analytical Toxicology, Vol. 32, No. 7, 2008, pp. 511-515.
[20] L. O. Henderson, M. K. Powell, W. H. Hannon, B. B. Miller, M. L. Martin, R. L. Hanzlick, D. Vroon and W. R. Sexson, “Radioimmunoassay Screening of Dried Blood Spot Materials for Benzoylecgonine,” Journal of Analytical Toxicology, Vol. 17, No. 1, 1993, pp. 42-47.
[21] L. O. Henderson, M. K. Powell, W. H. Hannon, J. T. Bernert, K. A. Pass, P. Fernhoff, C. D. Ferre, L. Martin, E. Franko, R. W. Rochat, M. D. Brantley and E. Sampson, “An Evalution of the Use of Dried Blood Spots from Newborn Screening for Monitoring the Prevalence of Cocaine Use among Childbearing Women,” Biochemical and Molecular Medicine, Vol. 61, No. 2, 1997, pp. 143- 151. doi:10.1006/bmme.1997.2609
[22] T. Wong, C. H. Shackleton, T. R. Covey and G. Ellis, “Identification of the Steroids in Neonatal Plasma that Interfere with 17 Alpha-Hydroxyprogesterone Radioimmunoassay,” Clinical Chemistry, Vol. 38, 1992, pp. 387- 395.
[23] G. La Marca, S. Malvagia, L. Fillipi, F. Luceri, G. Moneti and R. Guerrini, “A New Rapid Micromethod for the Assay of Phenobarbital from Dried Blood Spots by LC- Tandemmass Spectrometry,” Epilepsia, Vol. 50, No. 12, 2009, pp. 2658-2662. doi:10.1111/j.1528-1167.2009.02204.x
[24] R. G. Boy, J. Henseler, R. Mattern and G. Skopp, “Determination of Morphine and 6-Acetylmorphine in Blood with Use of Dried Blood Spots,” Therapeutic Drug Monitoring, Vol. 30, No. 6, 2008, pp. 733-739. doi:10.1097/FTD.0b013e31818d9fdb
[25] H. Schutz, J. C. Gotta, M. R. Erdmann and G. Weiler, “Simultaneous Screening and Detection of Drugs in Small Blood Samples and Blood Stains,” Forensic Sciences International, Vol. 126, No. 3, 2002, pp. 191-196. doi:10.1016/S0379-0738(02)00053-1
[26] C. S. Sosnoff, Q. Ann, J. T. Bernert Jr., M. K. Powell, B. B. Miller, L. O. Henderson, W. H. Hannon, P. Fernhoff and E. J. Sampson, “Analysis of Benzoylecgonine in Dried Blood Spots by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry,” Journal of Analytical Toxicology, Vol. 20, No. 3, 1996, pp. 179-184.
[27] L. G. Spector, S. S. Hecht, S. Ognjanovic and S. G. Carmella, “Detection of Cotinine in Newborn Dried Blood Spots,” Cancer Epidemiology, Biomarkers and Prevention, Vol. 16, 2007, pp. 1902-1905. doi:10.1158/1055-9965.EPI-07-0230
[28] US Department of Health and Human Services, Food and Drug Administration, “Guidance for Industry: Bioanalytical Method Validation,” Rockville, 2001. http://www.fda.gov/CDER/GUIDANCE/4252fnl.htm
[29] A. Diamandi, M. J. Khosravi, J. Mistry, V. Martinez and J. G. Aguirre, “Filter Paper Blood Spot Assay of Human Insulin like Growth Factor I (IGF-I) and IGF-Binding Protein-3 and Preliminary Application in the Evaluation of Growth Hormone Status,” Journal of Clinical Endocrinology and Metabolism, Vol. 83, No. 7, 1998, pp. 2296-2301. doi:10.1210/jc.83.7.2296

  
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