Use of radiation in strains of Saccharomyces cerevisiae: A new technique for industrial applications


During the industrial fermentation process in the production of fuel ethanol, yeasts are subject to several stressing conditions. The survival and the permanence of strains introduced in the process correlate with the capability of these yeasts in resisting to physical and chemical stresses, as well as their recovering ability to compete with contaminating micro-organisms commonly present in this industrial process. We aim at the selection of Saccharomyces cere visiae strains having this capability and ability. In this sense, cultivations of strains with industrial interest were irradiated with gammas ray at a wide dose interval. Growing curves for the strains were analyzed by means of their relative growth, a new concept here introduced, which allows a better understanding of the growing and recovering processes following radiative stress. It was found that gamma radiation could be used as an alternative method to quantify growing capabilities of S. cerevisiae strains under stressing conditions. It was also shown that this radiological method could be utilized as an additional procedure to select best robust industrial strains. This radiological method simplifies traditional analysis of strain viability, by avoiding the great number of necessary and consecutive fermentation assays.

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Andrette, R. , Arruda-Neto, J. , Basso, T. , Basso, L. , Cavalcante-Silva, E. , Bittencourt-Oliveira, M. and Genofre, G. (2013) Use of radiation in strains of Saccharomyces cerevisiae: A new technique for industrial applications. Advances in Bioscience and Biotechnology, 4, 346-351. doi: 10.4236/abb.2013.43045.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Abbott, D.A., Hynes, S.H., Ingledew, W.M. (2004) Growth rates of Dekkera/Brettanomyces yeasts hinder their ability to compete with Saccharomyces cerevisiae in batch corn mash fermentation. Applied Microbiology and Biotechnology, 66, 641-647.
[2] Silva-Filho, E.A., dos Santos, S.K.B., Resende, A.M., de Moraes, J.O.F., Morais Jr., M.A. and Sim?es, D.A. (2005) Yeast population dynamics of industrial fuel ethanol fermentation process assessed by PCR-fingerprinting. Antonie van Leeuwenkoek, 88, 13-23. doi:10.1007/s10482-004-7283-8
[3] Basso, L.C., Amorim, H.V., Oliveira, A.J. and Lopes, M.L. (2008) Yeast selection for fuel ethanol production in Brazil. FEMS Yeast Research, 8, 1155-1163. doi:10.1111/j.1567-1364.2008.00428.x
[4] Wheals, A.E., Basso, L.C., Alves, D.M.G. and Amorim, H.V. (1999) Fuel ethanol after 25 years. Trends in Bio- technology, 17, 482-487. doi:10.1016/S0167-7799(99)01384-0
[5] Basso, L.C., Paulilo, S.C.L., Rodrigues, D.A., Basso, T. O., Amorin, A.V. and Walker, G.M. (2004) Aluminium toxicity towards yeast fermentation and the protective effect of magnesium. International Congress on Yeasts—Yeasts in Science and Biotechnology The Quest for Sustainable Development, Book of Abstract, Rio de Janeiro, PB14.
[6] Dorta, C., Oliva-Neto, P., Abreu-Neto, M.S., Nicolu-Junior, N. and Nagashima, A.I. (2006) Synergism among lactic acid, sulfite, pH and ethanol in alcoholic fermentation of Saccharomyces cerevisiae (PE-2 and M-26). World Journal of Microbiology & Biotechnology, 22, 177-182. doi:10.1007/s11274-005-9016-1
[7] Barszczewski, W. and Robak, M. (2004) Differentiation of contaminating yeasts in brewery by PCR-based techniques. Food Microbiology, 21, 227-231. doi:10.1016/S0740-0020(03)00071-6
[8] Argueso, J.L., Carazzolle, M.F., Mieczkowski, P.A., Duarte, F.M., Netto, O.V., Missawa, S.K., Galzerani, F., Costa, G.G., Vidal, R.O., Noronha, M.F., Dominska, M., Andrietta, M.G., Andrietta, S.R., Cunha, A.F., Gomes, L.H., Tavares, F.C., Alcarde, A.R., Dietrich, F.S., McCusker, J.H., Petes, T.D. and Pereira, G.A. (2009) Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production. Genome Research, 19, 2258-2270. doi:10.1101/gr.091777.109
[9] Lodish, H., Berk, A., Matsudaira, P., Kaiser, C.A., Krieger, M., Scott, M.P., Zipursky, L. and Darnell, J. (1999) Molecular cell biology. 4th Edition, Freeman & Co., New York.
[10] Alpen, E.L. (1990) Radiation chemistry. In: Alpen, E.L., Ed., Radiation Biophysics, Editora Prentice-Hall do Brasil, Rio de Janeiro.
[11] Caria, M. (2000) Measurement analysis. Imperial College Press, London.
[12] James, A.P. and Werner, M.M. (1969) Multiple-step recovery from heritable lethal sectoring in yeast. Genetics, 62, 533-541.
[13] James, A.P. and Nasim, A. (1987) Effects of radiation on yeast. In: Rose, A.H. and Harrsison, J.S., Eds., The Yeasts, 2nd Edition, Vol. 2, Academic Press, New York.
[14] Mortimer, R.K. and Johnston, J.R. (1886) Genealogy of principal strains of the yeast genetic stock center. Genetics, 113, 35-43.
[15] Hall, E.J. (2006) Physics and chemistry of radiation absorption. In: Hall, E.J. and Giaccia, A.J., Eds., Radiobiology for the Radiologist, 6th Edition, J.B. Lippincott Company, New York.
[16] Alpen, E.L. (1990) Theories and models for cell survival. In: Alpen, E.L., Ed., Radiation Biophysics. Editora Prentice-Hall do Brasil, Rio de Janeiro.

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