Determination of amino-acidic positions important for Ocimum basilicum geraniol synthase activity

DOI: 10.4236/abb.2013.42033   PDF   HTML   XML   4,121 Downloads   6,894 Views   Citations


Terpenes are one of the largest and most diversified families of natural compounds. Although they have found numerous industrial applications, the molecular basis of their synthesis in plants has, until now, not been fully understood. Plant genomes have been shown to contain dozens of terpene synthase (TPS) genes, however knowledge of their amino-acidic protein sequence in not sufficient to predict which terpene(s) will be produced by a particular enzyme. In order to investigate the structural basis of a TPS specificity, we performed site directed mutations in the geraniol synthase from Ocimum basilicum. The results obtained suggest that a specific region on the catalytic site plays an important role in GPP transformation, either by stabilizing the GPP substrate on the catalytic site, or by enabling its transformation into a monoterpenol via an intermediate carbocation.

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Fischer, M. , Meyer, S. , Claudel, P. , Steyer, D. , Bergdoll, M. and Hugueney, P. (2013) Determination of amino-acidic positions important for Ocimum basilicum geraniol synthase activity. Advances in Bioscience and Biotechnology, 4, 242-249. doi: 10.4236/abb.2013.42033.

Conflicts of Interest

The authors declare no conflicts of interest.


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