Author(s)

Yongxiu Yao¹, Chan Ding², Venugopal Nair^1,3,4*

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Surrey, UK.
²Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
³The Jenner Institute Laboratories, University of Oxford, Oxford, UK.
⁴Department of Zoology, University of Oxford, Oxford, UK.

Herpesviruses account for most of the known virus-encoded miRNAs. Herpesvirus of turkey (HVT), a non-pathogenic avian herpesvirus used as an avian vaccine and viral vector, encodes 28 mature miRNAs. This included HVT-miR-H14-3p that showed almost identical sequence to gga-miR-221, suggesting that it is pirated from the avian host. Although the functional homolog between the two miRNAs has been proposed based on the sequence similarity, the direct experimental evidence is still lacking. In this report, we provide the evidence for the first time that HVT-miR-H14-3p is indeed a gga-miR-221 homolog through modulating the expression of p27^Kip1, a known target of miR-221 by binding to its 3’UTR. We also created an HVT-miR-H14-3p deletion virus and show that this miRNA is not essential for in vitro replication.

HVT, HVT-miR-H14-3p, Homolog, gga-miR-221, p27^Kip1

Yao, Y. , Ding, C. and Nair, V. (2019) Herpesvirus of Turkeys (Meleagridis Herpesvirus 1) Encodes a Functional MicroRNA-221 Homolog with High Sequence Conservation. Advances in Microbiology, 9, 728-736. doi: 10.4236/aim.2019.98044.